G-CSFR/CD114 Antibody (680206) [Unconjugated]

Recombinant Mouse G‑CSF (Catalog # 414-CS) stimulates proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse G‑CSF (0.15 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse G‑CSF R/CD114 Monoclonal Antibody (Catalog # MAB6039). The ND50 is typically 0.015‑0.075 µg/mL.

• Reactivity: Mu
• Applications: B/N
• Clone: 680206
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

G-CSFR/CD114 Antibody (680206) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse G-CSF R/CD114
  Cys26-Asp626
  Accession # P40223
• Specificity: Detects mouse G-CSF R/CD114 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) G-CSF R, rhGM‑CSF R beta, recombinant mouse (rm) GM-CSF R alpha, or rmM-CSF R is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: Csf3r
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.015-0.075 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for G-CSFR/CD114 Antibody (680206) [Unconjugated]

• CD114 antigen
• CD114
• colony stimulating factor 3 receptor (granulocyte)
• CSF3R
• Csfgr
• G-CSF R
• G-CSF receptor
• GCSFR
• G-CSFR
• GCSFRG-CSF-R
• granulocyte colony-stimulating factor receptor

Background

Granulocyte colony stimulating factor (G-CSF) is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic and granulocyte lineage (1). G-CSF plays an important role in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Cell activation by G‑CSF is mediated by granulocyte colony stimulating factor receptor alpha (G-CSF R; also CD114), a 95‑105 kDa type I transmembrane protein and member of the cytokine receptor superfamily, type I cytokine receptor family, and type 2 subfamily of receptor proteins. Mouse G‑CSF R is synthesized as an 837 amino acid (aa) precursor that contains a 25 aa signal sequence, a 601 aa extracellular domain (ECD), a 24 aa transmembrane region, and a 187 aa cytoplasmic tail. The ECD contains one Ig-like C2-type domain, five fibronectin type-III domains, and 11 potential sites for N‑linked glycosylation. Within the ECD there is also a WSXWS motif (aa 319‑323) that is necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding (2). Also, within the cytoplasmic domain there is a Box 1 motif which is required for JAK interaction and/or activation (1). Mouse G‑CSF R shares 63% aa sequence identity with human G‑CSF R. G-CSF R is expressed in mature neutrophils, neutrophilic precursors, myeloid leukemia cells, and placenta (1). Mutations have been found in the gene encoding G-CSF R in some patients with severe congenital neutropenia (1). These mutations typically lead to a truncation in the cytoplasmic domain of the G-CSF R leading to maturation arrest of neutrophilic precursors in the bone marrow and neutropenia in peripheral blood (3). Binding of G-CSF to its receptor induces dimerization or oligomerization of the receptor activating cytoplasmic tyrosine kinases (2). Signal transduction from pathways that involve Janus tyrosine kinases/signal transducer and activator of transcription proteins (Jak1, Jak2, and Tyk2/STAT3 and STATG), src-related protein tyrosine kinases (Lyn and Syk), Ras/MAP kinase, and phosphatidylinositol have been reported to be activated upon G-CSF stimulation (4).

1. Ward, A.C. (2007) Front. Biosci. 12:608.   
2. Layton, J.E. and N.E. Hall (2006) Front. Biosci. 11:3181.
3. Mitsui, T. et al. (2003) Blood 101:2990.
4. Nicola, N.A. in Cytokine Reference, 2001, Oppenheim, J.J. and M. Feldmann, eds. Academic Press p.1935.

 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for G-CSFR/CD114 Antibody (MAB6039)(6)

Publications using MAB6039

• M Takehara, Y Sonobe, H Bandou, K Kobayashi, M Nagahama Granulocyte Colony-Stimulating Factor Does Not Influence Clostridium Perfringens α-Toxin-Induced Myonecrosis in Mice Toxins (Basel), 2019-08-30;11(9):. 2019-08-30 [PMID: 31480318] (Neutralization, Mouse)
• Thomas Riffelmacher, Alexander Clarke, Felix C. Richter, Amanda Stranks, Sumeet Pandey, Sara Danielli et al. Autophagy-Dependent Generation of Free Fatty Acids Is Critical for Normal Neutrophil Differentiation Immunity 9/19/2017 [PMID: 28916263]
• Thomas Riffelmacher, Alexander Clarke, Felix C. Richter, Amanda Stranks, Sumeet Pandey, Sara Danielli et al. Autophagy-Dependent Generation of Free Fatty Acids Is Critical for Normal Neutrophil Differentiation Immunity 2017-09-19 [PMID: 28916263] (Flow Cytometry, Mouse)
• LECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells Nat Commun, 2016-09-06;7(0):12719. 2016-09-06 [PMID: 27596364] (Neutralization, Mouse)
• D. Clark Files, Chun Liu, Andrea Pereyra, Zhong-Min Wang, Neil R. Aggarwal, Franco R. D’Alessio et al. Therapeutic exercise attenuates neutrophilic lung injury and skeletal muscle wasting Science Translational Medicine 3/11/2015 [PMID: 25761888]
• D. Clark Files, Chun Liu, Andrea Pereyra, Zhong-Min Wang, Neil R. Aggarwal, Franco R. D’Alessio et al. Therapeutic exercise attenuates neutrophilic lung injury and skeletal muscle wasting Science Translational Medicine 2015-03-11 [PMID: 25761888] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: Csf3r
• Uniprot:
  • P40223()