Fyn Antibody

Western Blot: Fyn Antibody [NB300-928] - Analysis of A431 (A) and Jurkat lysate (B) (35ug protein in RIPA buffer).

Western Blot: Fyn Antibody [NB300-928] - Analysis of Jurkat lysate (RIPA buffer, 35ug total protein per lane).

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Fyn Antibody Summary

• Description: Novus Biologicals Goat Fyn Antibody (NB300-928) is a polyclonal antibody validated for use in WB and ELISA. Anti-Fyn Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence VQCKDKEATKLTE corresponding to N-Terminus according to NP_694592.1, NP_694593.1.
• Specificity: This antibody is expected to recognise all three reported isoforms (as represented by NP_002028.1; NP_694592.1; NP_694593.1).
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FYN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 55 kDa band observed in lysates of A431 and Jurkat (calculated MW of 54.5 kDa band according to NP_694593.1).
• Control:

  Fyn Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Fyn Antibody

• EC 2.7.10
• EC 2.7.10.2
• FYN oncogene related to SRC, FGR, YES
• Fyn
• MGC45350
• OKT3-induced calcium influx regulator
• p59-Fyn
• protein-tyrosine kinase fyn
• Proto-oncogene c-Fyn
• Proto-oncogene Syn
• proto-oncogene tyrosine-protein kinase fyn
• SLK
• SLKc-syn protooncogene
• src/yes-related novel
• Src-like kinase
• SYN
• tyrosine kinase p59fyn(T)
• tyrosine-protein kinase Fyn

Background

This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fyn Antibody (NB300-928)(1)

Publication using NB300-928

• Semba K, Nishizawa M, Miyajima N et al. yes-related protooncogene, syn, belongs to the protein-tyrosine kinase family. Proc Natl Acad Sci U S A 2008-01-08 [PMID: 3526330]

Bioinformatics

• Gene Symbol: FYN
• Entrez:
  • 2534(Human)
  • 14360(Mouse)
  • 25150(Rat)
• Uniprot:
  • NP_002028(Human)