Fyn Antibody (361228) [Unconjugated]

Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line, C2C12 mouse myoblast cell line, CTLL-2 mouse cytotoxic T cell line, NIH-3T3 mouse embryonic fibroblast cell line, PT18 mouse mast/basophil cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Fyn Monoclonal Antibody (Catalog # MAB3574) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Fyn at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Fyn was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human/Mouse/Rat Fyn Monoclonal Antibody (Catalog # MAB3574) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: 361228
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Fyn Antibody (361228) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Fyn
  Met1-Gly83
  Accession # P06241
• Specificity: Detects endogenous human, mouse and rat Fyn in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FYN
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fyn Antibody (361228) [Unconjugated]

• EC 2.7.10
• EC 2.7.10.2
• FYN oncogene related to SRC, FGR, YES
• Fyn
• MGC45350
• OKT3-induced calcium influx regulator
• p59-Fyn
• protein-tyrosine kinase fyn
• Proto-oncogene c-Fyn
• Proto-oncogene Syn
• proto-oncogene tyrosine-protein kinase fyn
• SLK
• SLKc-syn protooncogene
• src/yes-related novel
• Src-like kinase
• SYN
• tyrosine kinase p59fyn(T)
• tyrosine-protein kinase Fyn

Background

Fyn is a ubiquitously expressed member of the Src family of intracellular tyrosine kinases. Fyn activity is implicated in T cell receptor signaling, adhesion-mediated signaling, and regulation of brain function. Mice lacking fyn display various deficits, including impaired spatial memory, hypomyelination, and uncoordinated hippocampal structure, suggesting that Fyn is involved in multiple neural signaling pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fyn Antibody (MAB3574)(4)

Publications using MAB3574

• William K. Kanagy, Cédric Cleyrat, Mohamadreza Fazel, Shayna R. Lucero, Marcel P. Bruchez, Keith A. Lidke et al. Docking of Syk to Fc epsilon RI is enhanced by Lyn but limited in duration by SHIP1 Molecular Biology of the Cell 2022-09-01 [PMID: 35793126]
• Westman J, Plumb J, Licht A et al. Calcium-dependent ESCRT recruitment and lysosome exocytosis maintain epithelial integrity during Candida albicans invasion Cell reports 2022-01-04 [PMID: 34986345]
• Masahiro Yasuda, Sivapratha Nagappan-Chettiar, Erin M. Johnson-Venkatesh, Hisashi Umemori An activity-dependent determinant of synapse elimination in the mammalian brain Neuron 2021-04-01 [PMID: 33770504]
• Masahiro Yasuda, Sivapratha Nagappan-Chettiar, Erin M. Johnson-Venkatesh, Hisashi Umemori An activity-dependent determinant of synapse elimination in the mammalian brain Neuron 2021-04-01 [PMID: 33770504] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: FYN
• Uniprot:
  • P06241()