FUS Antibody (JJ09-31)

Western Blot: FUS Antibody (JJ09-31) [NBP2-67913] - Analysis of FUS/TLS on K562 cells lysates using anti-FUS/TLS antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: FUS Antibody (JJ09-31) [NBP2-67913] - Staining FUS/TLS in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: FUS Antibody (JJ09-31) [NBP2-67913] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-FUS/TLS antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: FUS Antibody (JJ09-31) [NBP2-67913] - Analysis of Hela cells with FUS/TLS antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: FUS Antibody (JJ09-31) [NBP2-67913] - Staining FUS/TLS in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: FUS Antibody (JJ09-31) [NBP2-67913] - Staining FUS/TLS in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: FUS Antibody (JJ09-31) [NBP2-67913] - Analysis of paraffin-embedded human kidney tissue using anti-FUS/TLS antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: FUS Antibody (JJ09-31) [NBP2-67913] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-FUS antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: FUS Antibody (JJ09-31) [NBP2-67913] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-FUS antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

FUS Antibody (JJ09-31) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human aa 1-46 / 526. (SwissProt: P35637 Human; SwissProt: P56959 Mouse)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FUS
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for FUS Antibody (JJ09-31)

• 75 kDa DNA-pairing protein
• ALS6
• amyotrophic lateral sclerosis 6
• FUS1
• fused in sarcoma
• fusion (involved in t(12;16) in malignant liposarcoma)
• fusion gene in myxoid liposarcoma
• fusion, derived from t(12;16) malignant liposarcoma
• fus-like protein
• heterogeneous nuclear ribonucleoprotein P2
• HNRNPP2
• Oncogene FUS
• Oncogene TLS
• POMP75
• RNA-binding protein FUS
• TLShnRNP-P2
• Translocated in liposarcoma protein

Background

FUS, fused in sarcoma, encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Coactivates expression in an agonist- and AF2-dependent manner. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ERs), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Probably functions as a general coactivator, rather than just a nuclear receptor coactivator. May also be involved in the coactivation of the NF-kappa-B pathway. May coactivate expression via a remodeling of chromatin and its interaction with histone acetyltransferase proteins. Involved in placental, cardiac, hepatic and embryonic development. NCOA6 is over-expressed in several breast cancer cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FUS