FTO Antibody (729722)

Western blot shows lysates of U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FTO Monoclonal Antibody (Catalog # MAB7208) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FTO at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, IP
• Clone: 729722
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

FTO Antibody (729722) Summary

• Immunogen: E. coli-derived recombinant human FTO
  Met1-Arg178
  Accession # Q9C0B1
• Specificity: Detects human FTO in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FTO
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FTO Antibody (729722)

• Alpha-ketoglutarate-dependent dioxygenase FTO
• EC 1.14.11.-
• fat mass and obesity associated
• Fat mass and obesity-associated protein
• FTO
• KIAA1752protein fto
• MGC5149

Background

FTO (FATSO; also known as Fat Mass and Obesity Associated gene) is a 58 kDa member of the AlkB family of Ferrous/2-Oxoglutarate-dependent oxidative DNA/RNA demethylases. It is found the nucleus, and apparently serves to protect single stranded RNA and DNA against methylation. Although FTO is ubiquitously expressed, it has high levels in neurons that are involved in regulating energy intake, and is often found to colocalize with Oxytocin. Evidence suggests it promotes feeding behavior. Human FTO is 505 amino acids (aa) in length. It contains one dioxygenase catalytic region (aa 32-327) and a C-terminal FTO domain (aa 329-500). There are three potential isoform variants. One shows a ten aa substitution for aa 1-455, a second contains a 35 aa substitution for aa 1-413, and a third utilizes an alternative start site at Met400. Over aa 1-178, human FTO shares 89% aa identity with mouse FTO.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for FTO Antibody (MAB7208). (Showing 1 - 1 of 1 FAQ).

1. Do you have any antibodies against FTO, where you can indicate the sequence of the immunogen?
   • With regards to other FTO antibodies that we do list the peptide sequence for, the immunogen sequence for catalog number NB110-60935 can be found in the range of amino acids 400-505. Many of the exact immunogen sequences used for our products are proprietary and cannot be disclosed. In these instances we try to provide a reasonable amino acid range to help in your decision.

Bioinformatics

• Gene Symbol: FTO
• Uniprot:
  • Q9C0B1(Human)