Recombinant Human Frk Protein

SDS-Page: Recombinant Frk Protein [NBP1-99510]

Product Discontinued

Recombinant Human Frk Protein Summary

• Description: Recombinant protein for Human Frk
  
  

  Source:Baculovirus (Sf9 insect cells)
  Amino Acid Sequence:(NM_002031)

• Source: Sf 9 (baculovirus)
• Protein/Peptide Type: Recombinant Protein
• Gene: FRK
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Specific activity: The specific activity of FRK was determined to be 997 nmol /min/mg as per activity assay protocol.
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
• Concentration: 0.1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Frk Protein

• EC 2.7.10
• Frk
• fyn-related kinaseProtein-tyrosine kinase 5
• Gtk
• GTKEC 2.7.10.2
• Nuclear tyrosine protein kinase RAK
• PTK5 protein tyrosine kinase 5
• PTK5
• Rak
• RAKPTK5
• tyrosine-protein kinase FRK

Background

FRK (fyn-related kinase) or Rak is a nuclear tyrosine kinase and member of the Src sub-family. Restricted expression of FRK is detected in a broad range of cell lines with highest levels in epithelial cells. Increased expression of FRK has been shown in breast and renal cell carcinoma cell lines. In addition the retinoblastoma tumor susceptibility gene product pRb associates with FRK in vitro and in vivo (1). Overexpression of FRK in Beta -cells from the pancreas increases the susceptibility of these cells to Beta -cell-toxic events (hallmark of Type I diabetes)(2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: FRK