Frizzled-6 Antibody - BSA Free

Western Blot: Frizzled-6 Antibody [NBP3-18335] - WB Validation in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysate. Antibodies: Frizzled-6 NBP3-18335, 2 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.

Immunocytochemistry/ Immunofluorescence: Frizzled-6 Antibody - BSA Free [NBP3-18335] - Validation of FZD6 in Mouse Lung.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung labeling FZD6 with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunocytochemistry/ Immunofluorescence: Frizzled-6 Antibody - BSA Free [NBP3-18335] - Validation of FZD6 in Rat Lung. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung labeling FZD6 with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunocytochemistry/ Immunofluorescence: Frizzled-6 Antibody - BSA Free [NBP3-18335] - Validation of FZD6 in Daudi Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling FZD6 with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

• Reactivity: Hu, Mu, Rt, Ca, Pm, Pm
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Frizzled-6 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Frizzled-6 Antibody - BSA Free (NBP3-18335) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a peptide corresponding to 14 amino acids in the central domain of human Frizzled-6. The immunogen is located within 50-550 amino acids of Frizzled-6.
• Predicted Species: Chimpanzee (100%), Monkey (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FZD6
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Western Blot 2 ug/mL
• Control Peptide:

  Frizzled-6 Protein (NBP3-18335PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Frizzled-6 Antibody - BSA Free

• frizzled (Drosophila) homolog 6
• frizzled homolog 6 (Drosophila)
• Frizzled6
• Frizzled-6
• FZ6
• fz-6
• FZD6
• Hfz6
• seven transmembrane helix receptor

Background

FZD6 is a Frizzled Receptor for Wnt signaling proteins involved in patterning during development. Contrary to other Frizzled proteins, FZD6 does not contain a C-terminal PDZ domain-binding motif. FZD6 is widely expressed. The Drosophila homolog activates Dishevelled. FZD6 expression has been reported in brain, colon, heart, kidney, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, testis, and thymus. Various tumor cell lines have also been shown to express FZD6. ESTs have been isolated from blood, brain, breast, connective tissue, liver, liver/spleen, lung, placenta, testis, thyroid, and vessel libraries. In addition, several ESTs have been isolated from tumor libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FZD6