Frizzled-5 Antibody [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

Frizzled-5 Antibody [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Frizzled‑5
  Ala27-Pro167
  Accession # AAC50385
• Specificity: Detects human Frizzled-5 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) Frizzled-1, rmFrizzled-2, rmFrizzled-3, rmFrizzled-6, rmFrizzled-7, and rmFrizzled-8 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Frizzled-5 Antibody [Alexa Fluor® 488]

• C2orf31
• chromosome 2 open reading frame 31
• DKFZP434E2135
• frizzled (Drosophila) homolog 5
• frizzled homolog 5 (Drosophila)
• Frizzled5
• Frizzled-5
• Frz-5
• FZ-5
• FZD5
• fzE5
• HFZ5
• MGC129692
• seven-transmembrane receptor frizzled-5
• Wnt receptor

Background

Wnt signaling is involved in variety of developmental processes including cell fate determination, cell polarity, tissue patterning and control of cell proliferation. Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway. The founding member of this family was identified in Drosophila based on its role in tissue polarity in the adult cuticle and named for the disorganized appearance of bristle hairs on the mutant. The predicted structure of Frizzled proteins is similar among all family members, containing a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain, a variable-length linker region, a seven-pass transmembrane domain, and a variable-length C-terminal tail. One of the most conserved regions of the Frizzled (Frz) proteins is the extracellular cysteine-rich domain (CRD) which spans approximately 120 amino acid (aa) and contains 10 invariant cysteines (1). Human Frz-5 shows 95% aa identity to mouse Frz-5 in the CRD region. In the mouse, Frz-5 is expressed in adult tissues (heart and kidney), as well as embryonic tissues (telencephalon, eye, and lung bud) (2, 3). Null mutations in Frz-5 reveal that it plays a role in the formation and maintenance of the extra-embryonic vasculature (3). Functional interactions with Frz-5 have been reported for Wnt-5a, Wnt-10b, Wnt-2b, and Wnt-7a, implicating these Wnts as ligands for the Frz-5 receptor (3-5).

Two distinct Wnt signal transduction pathways have been characterized. One is the canonical Wnt/ beta -catenin pathway that is involved in diverse biological mechanisms such as dorsal/ventral development in Xenopus embryos and mammalian tumor formation. Frz-5 is implicated in this pathway based on its ability to induce beta -catenin target genes in the presence of ligand (5). However, Frz-5 is also implicated in beta -catenin independent pathways (4, 6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.