Frizzled-3 Antibody - BSA Free

Immunohistochemistry-Paraffin: Frizzled-3 Antibody [NBP3-14325] - Immunohistochemistry of formalin-fixed, paraffin-embedded human skin after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: Frizzled-3 Antibody [NBP3-14325] - Immunohistochemistry of formalin-fixed, paraffin-embedded human skeletal muscle after heat-induced antigen retrieval.

• Reactivity: Hu, Mu, Rt, Po, Av, Bt, Bv, Ca, Ch, Eq, Ha, Pm, Ma-Op, Pm, Rb
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Frizzled-3 Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for up to 1 month.
• Immunogen: Synthetic 18 amino acid peptide from N-Terminus extracellular domain of human Frizzled-3.
• Epitope: N-Terminus
• Specificity: Human Frizzled-3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Rabbit (100%), Bovine (100%), Opossum (100%), Avian (100%), Hamster (100%), Chicken (100%), Canine (100%), Bat (100%), Monkey (100%), Equine (100%), Primate (100%). Backed by our 100% Guarantee.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FZD3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 3-5 ug/ml..

Reactivity Notes

Expected reactivity based on percent sequence identity: Xenopus (89%), Zebrafish (83%)

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Frizzled-3 Antibody - BSA Free

• frizzled (Drosophila) homolog 3
• frizzled homolog 3 (Drosophila)
• Frizzled3
• Frizzled-3
• Fz-3
• FZD3
• hFz3

Background

Frizzled-3 is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. The function of this protein is unknown, although it may play a role in mammalian hair follicle development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FZD3