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Frizzled-1 Antibody (162531) [Unconjugated]

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HEK293 human embryonic kidney cell line transfected with Frizzled-1 was stained with Rat Anti-Human/Mouse Frizzled-1 Monoclonal Antibody (Catalog # MAB11201, filled histogram) or isotype control antibody (Catalog # ...read more
HEK293 human embryonic kidney cell line was stained with Rat Anti-Human/Mouse Frizzled-1 Monoclonal Antibody (Catalog # MAB11201, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clone
162531
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Frizzled-1 Antibody (162531) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Frizzled‑1
Gln72-His248 (Met122Ile)
Accession # O70421
Specificity
Detects mouse Frizzled-1 in direct ELISAs and Western blots. Detects human Frizzled-1 in Flow Cytometry. In direct ELISAs, approximately 30% cross‑reactivity with recombinant mouse (rm) Frizzled-9 is observed and no cross-reactivity with recombinant human (rh) Frizzled-5, rmFrizzled 2, 3, 4, 6, 7, 8, or rhMFRP is observed. No staining is observed on normal human PBMCs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
FZD1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB11201 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Frizzled-1 Antibody (162531) [Unconjugated]

  • DKFZp564G072
  • FLJ95923
  • frizzled family receptor 1
  • frizzled homolog 1
  • frizzled, Drosophila, homolog of, 1
  • Frizzled1
  • Frizzled-1
  • fz-1
  • FZD1
  • fzE1
  • hFz1
  • seven transmembrane spanning receptor
  • Wnt receptor

Background

The Wnt genes encode a large family of glycoproteins that are essential in development and tissue maintenance (1). Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway (2). Ten mouse and human Frizzled genes have been identified to date. The predicted structure of Frizzled proteins is similar among all family members, containing a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain (CRD), a variable-length linker region, a seven-pass transmembrane region, and a variable-length C-terminal cytoplasmic domain. The CRD comprises 642 amino acids and shares 95% identity with the human orthologue. Frizzled-1 mRNA has been detected in relatively large amounts in adult heart, placenta, lung, kidney, pancreas, prostate, and ovary, and in fetal lung and kidney (3). Several Frizzled-dependent signaling pathways exist (2). Their activation depends on the Wnt ligand and the cell context. Members of the low density lipoprotein receptor-related protein (LRP) are co-receptors for the Wnt ligands. LRP5/6 serve as co-receptors in the Wnt/Frizzled canonical pathway that alters gene expression via the stabilization of beta -catenin (4, 5). LRP1 may down regulate Wnt-3a/Frizzled-1 signaling in the canonical pathway by sequestering Frizzled-1 (6). Frizzled-1 is one of the purported Wnt-10b receptors whose signaling inhibits adipogenesis in preadipocytes (7). Frizzled-1 may be part of a feedback mechanism to modulate the effects of BMP-2 in mesenchymal cells since upregulation of its expression by BMP-2 counteracts the effects of BMP-2 and Wnt-3a in inducing the expression of the osteoblast differentiation marker, alkaline phosphatase (8).
  1. Wodarz, A. and R. Nusse (1998) Annu. Rev. Cell Dev. Biol. 14:59. 
  2. Hsieh, J-C. (2004) Front. Biosci., 9:1333. 
  3. Xu, L. et al. (2001) Matrix Biol. 2:147. 
  4. Tamai, K. et al. (2000) Nature 407:530. 
  5. Pinson, K. et al. (2000) Nature 407:535.
  6. Zielberberg, A. et al. (2004) J. Biol. Chem. 279:17535.
  7. Bennet, C. et al. (2002) J. Biol. Chem. 277:30998.
  8. Roman-Roman, S. et al. (2004) J. Biol. Chem. 279:5725.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Frizzled-1 Antibody (MAB11201)(2)

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Bioinformatics

Gene Symbol FZD1
Uniprot