FPRP/PTGFRN Antibody (998175) [Unconjugated]

Western blot shows lysates of LoVo human colorectal adenocarcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, C2C12 mouse myoblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat FPRP/PTGFRN Monoclonal Antibody (Catalog # MAB10043) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FPRP at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

FPRP/PTGFRN was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human FPRP/PTGFRN Monoclonal Antibody (Catalog # MAB10043) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 998175
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

FPRP/PTGFRN Antibody (998175) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell, HEK293-derived human FPRP/PTGFRN
  Pro27-Pro832
  Accession # Q9P2B2
• Specificity: Detects human FPRP/PTGFRN in direct ELISAs. Detects human, mouse, and rat FPRP/PTGFRN in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FPRP/PTGFRN Antibody (998175) [Unconjugated]

• CD315
• CD9P-1
• FPRP
• PTGFRN

Background

FPRP (F_{2 alpha} Prostaglandin receptor Regulatory Protein; also CD315, EWI-F and CD9 partner-1/P-1) is a 130 kDa type I transmembrane (TM) glycoprotein member of the EWI subfamily, Ig superfamily of molecules. It associates with the 7-TM prostaglandin F_{2 alpha}  receptor, inhibiting its activity. It also forms complexes with 4-TM CD9 and CD81, linking these molecules to the intracellular actin cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FPRP/PTGFRN Antibody (MAB10043)(1)

Publication using MAB10043

• Mattia I Morandi, Petro Busko, Efrat Ozer-Partuk, Suman Khan, Giulia Zarfati, Yael Elbaz-Alon et al. Extracellular vesicle fusion visualized by cryo-electron microscopy PNAS Nexus 2022-09-01 [PMID: 36714848]

Bioinformatics

• Uniprot:
  • Q9P2B2(Human)