FPRP/PTGFRN Antibody (998107) [Unconjugated]

U87-MG human glioblastoma cell line was stained with Mouse Anti-Human FPRP/PTGFRN Monoclonal Antibody (Catalog # MAB100431, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 998107
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

FPRP/PTGFRN Antibody (998107) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell, HEK293-derived human FPRP/PTGFRN
  Pro27-Pro832
  Accession # Q9P2B2
• Specificity: Detects human FPRP/PTGFRN in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FPRP/PTGFRN Antibody (998107) [Unconjugated]

• CD315
• CD9P-1
• FPRP
• PTGFRN

Background

FPRP (F_{2 alpha} Prostaglandin receptor Regulatory Protein; also CD315, EWI-F and CD9 partner-1/P-1) is a 130 kDa type I transmembrane (TM) glycoprotein member of the EWI subfamily, Ig superfamily of molecules. It associates with the 7-TM prostaglandin F_{2 alpha}  receptor, inhibiting its activity. It also forms complexes with 4-TM CD9 and CD81, linking these molecules to the intracellular actin cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FPRP/PTGFRN Antibody (MAB100431)(1)

Publication using MAB100431

• A Timpanaro, C Piccand, AC Uldry, PK Bode, D Dzhumashev, R Sala, M Heller, J Rössler, M Bernasconi Surfaceome Profiling of Cell Lines and Patient-Derived Xenografts Confirm FGFR4, NCAM1, CD276, and Highlight AGRL2, JAM3, and L1CAM as Surface Targets for Rhabdomyosarcoma International Journal of Molecular Sciences, 2023-01-30;24(3):. 2023-01-30 [PMID: 36768928] (Flow Cytometry, Human)

Bioinformatics

• Uniprot:
  • Q9P2B2(Human)