FOXO3 [p Ser253] Antibody

Images

 
Western Blot: FOXO3 [p Ser253] Antibody [NB100-81929] - Analysis of extracts from NIH/3T3 cells using FKHRL1 (Ab-253) antibody (Lane 1 and 2) and FKHRL1 (phospho-Ser253) antibody (Lane 3 and 4).
Immunocytochemistry/ Immunofluorescence: FOXO3 [p Ser253] Antibody [NB100-81929] - Staining of methanol-fixed HeLa cells using FKHRL1 (phospho-Ser253) antibody.
Immunohistochemistry-Paraffin: FOXO3 [p Ser253] Antibody [NB100-81929] - Analysis of paraffin-embedded human breast carcinoma tissue using FKHRL1 (phospho-Ser253) antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

FOXO3 [p Ser253] Antibody Summary

Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human FKHRL1 around the phosphorylation site of serine 253 (A-V-SP-M-D).
Modification
p Ser253
Specificity
FKHRL1 (specific to Phospho-Ser253) detects endogenous levels of FKHRL1 only when phosphorylated at serine 253.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FOXO3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
FOXO3 Lysate (NBL1-10814)

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FOXO3 [p Ser253] Antibody

  • AF6q21 protein
  • AF6q21
  • DKFZp781A0677
  • FKHRL1
  • FKHRL1MGC31925
  • FKHRL1P2
  • forkhead box O3
  • forkhead box protein O3
  • forkhead homolog (rhabdomyosarcoma) like 1
  • Forkhead in rhabdomyosarcoma-like 1
  • forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
  • FOXO2
  • FoxO3
  • FOXO3A
  • FOXO3Aforkhead box O3A
  • MGC12739

Background

FOXO3A belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FOXO3 Antibody (NB100-81929) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FOXO3
Entrez
Uniprot