FOXO3 Antibody - BSA Free

Western Blot: FOXO3 Antibody [NB100-613] - Detection of Human and Mouse FOXO3a by Western Blot. Samples: Whole cell lysate (50 ug) from 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-FOXO3a antibody NB100-613 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry: FOXO3 Antibody [NB100-613] - Sample: FFPE sections of human ovarian carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-fs18 FOXO3a fs20 used at a dilution of 1:500 (2ug/ml). Detection: DAB

Western Blot: FOXO3 Antibody [NB100-613] - Detection of Human FOXO3a on HeLa whole cell lysate using NB100-613. FOXO3a was also immunoprecipitated using NB100-614.

Immunoprecipitation: FOXO3 Antibody [NB100-613] - Detection of human FOXO3a by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from MCF-7 cells. Antibodies: Affinity purified rabbit anti-FOXO3a antibody NB100-613 used for IP at 6 ug/mg lysate. FOXO3a was also immunoprecipitated by rabbit anti-FOXO3a antibody NB100-614. For blotting immunoprecipitated FOXO3a, NB100-613 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

FOXO3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit FOXO3 Antibody - BSA Free (NB100-613) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-FOXO3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residues 250 and 300 of human Forkhead Box O3a using the numbering given in Swiss-Prot entry O43524 (GeneID 2309).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FOXO3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:5000-1:15000
• Application Notes: Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
• Control:

  FOXO3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FOXO3 Antibody - BSA Free

• AF6q21 protein
• AF6q21
• DKFZp781A0677
• FKHRL1
• FKHRL1MGC31925
• FKHRL1P2
• forkhead box O3
• forkhead box protein O3
• forkhead homolog (rhabdomyosarcoma) like 1
• Forkhead in rhabdomyosarcoma-like 1
• forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
• FOXO2
• FoxO3
• FOXO3A
• FOXO3Aforkhead box O3A
• MGC12739

Background

The FOXO3 gene is known to promote longevity in animals and has now been discovered to encode a tumor suppressor. The new gene, which was inactivated in two-thirds of patients studied, presents a potent new target for breast cancer. The protein, named Forkhead box class O3 (FOXO3), is associated with better outcomes for breast cancer patients. Conversely, if FOXO3 was inactivated, patients had worse outcomes. FOXO3 is inactivated in many cancer patients by an enzyme called IKK, which is an oncoprotein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FOXO3 Antibody (NB100-613)(1)

Publication using NB100-613

• Yuan J, Luo K, Liu T et al. Regulation of SIRT1 activity by genotoxic stress. Genes Dev 2012-04-01 [PMID: 22465953]

Bioinformatics

• Gene Symbol: FOXO3
• Entrez:
  • 2309(Human)
• Uniprot:
  • O43524(Human)