FOXO1A/3/4 Antibody (4E7) - Azide and BSA Free

Immunohistochemistry: FOXO1A/3/4 Antibody (4E7) [NBP1-41196]

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 4E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

FOXO1A/3/4 Antibody (4E7) - Azide and BSA Free Summary

• Immunogen: Amino acids 242-655 of FKHR expressed in E. coli.
• Specificity: This antibody will recognize human FKHR.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FOXO1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10 - 1:500
  • Western Blot 0.1-2 ug/ml
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for FOXO1A/3/4 Antibody (4E7) - Azide and BSA Free

• FKH1
• FKHRforkhead box protein O1
• forkhead box O1
• Forkhead box protein O1A
• forkhead homolog in rhabdomyosarcoma
• Forkhead in rhabdomyosarcoma
• FOXO1Aforkhead, Drosophila, homolog of, in rhabdomyosarcoma

Background

FKHR-7H3 recognizes FKHR (ForKHead in Rhabdomyosarcoma), a 70a protein, which is a member of the forkhead homeotic gene family of transcription factors (FKH/HNF-3). Members of the forkhead family can be classified and grouped by their degree of homology within the 100-120aa region that is necessary and sufficient for DNA binding and binding-site specificity. The FKHR subfamily of forkhead proteins is relatively divergent from the other members of the forkhead transcription factor family. With all members in this subfamily containing a signature insertion of 5 amino acids next to the helix 3 in the middle of the forkhead domain, and lack the well conserved amino terminal KPPY sequence found in most forkhead genes. Recently, it has been shown that members of the FKHR gene family are phosphorylated by Akt and this phosphorylation regulates its cellular localization promoting cell survival.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FOXO1
• Entrez:
  • 2308(Human)