FoxK1 Antibody

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Western blot shows lysates of A172 human glioblastoma cell line. PVDF Membrane was probed with 1 µg/mL of Human FoxK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6366) followed by HRP-conjugated ...read more
FoxK1 was detected in immersion fixed paraffin-embedded sections of human colon using Human FoxK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6366) at 10 µg/mL overnight at 4 °C. Before incubation with ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

FoxK1 Antibody Summary

Immunogen
E. coli-derived recombinant human FoxK1
Lys493-Lys670
Accession # P85037
Specificity
Detects human FoxK1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse FoxK1 is observed and less than 5% cross-reactivity with recombinant human (rh) FoxK2 and rhFoxJ1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FOXK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FoxK1 Antibody

  • FLJ14977
  • forkhead box K1
  • forkhead box protein K1
  • FoxK1
  • FOXK1L
  • IMAGE:5164497
  • MNF
  • Myocyte nuclear factor

Background

FOX K1 (Forkhead box protein K1; also MNF) is a 75 kDa (predicted) nuclear protein that is a Class 1 member of the FOX family of transcription factors. It should not be confused with FOXK2/ILF1 that has also been referred to as FOXK1. It is widely expressed, but has become known as a marker for skeletal muscle satellite cells in the adult. Human FOXK1 is 733 amino acids (aa) in length. It contains one FHA domain that binds phosphopeptides (aa 116-203), plus a forkhead DNA binding region (aa 305-400) and over a dozen Ser/Thr phosphorylation sites. There are multiple potential splice variants. Two utilize an alternative start site at Met118 that may be accompanied by a 28 aa substitution for aa 673-733, while another contains 24 aa substitution for aa 1-187. Over aa 493-670, human FOXK1 shares 90% aa identity with mouse FOXK1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol FOXK1
Entrez
Uniprot