FoxJ1/HFH4 Antibody (407003) [Unconjugated]

HEK293 human embryonic kidney cell line was stained with Rat Anti-Human FoxJ1 Monoclonal Antibody (Catalog # MAB3619, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

• Reactivity: Hu
• Applications: CyTOF-ready, Flow
• Clone: 407003
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

FoxJ1/HFH4 Antibody (407003) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human FoxJ1
  Lys306-Leu421
  Accession # Q92949.3
• Specificity: Detects human FoxJ1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: FOXJ1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FoxJ1/HFH4 Antibody (407003) [Unconjugated]

• FKHL13
• FKHL13forkhead transcription factor HFH-4
• forkhead box J1
• forkhead-like 13
• Forkhead-related protein FKHL13
• FoxJ1
• Hepatocyte nuclear factor 3 forkhead homolog 4
• HFH-4
• HFH4fork head homologue 4
• HFH-4forkhead box protein J1
• MGC35202

Background

Human FoxJ1 (also HNF-4) is a 45 kDa, class 1 member of the HNF-3/fork-head gene family of transcription factors. It is 421 amino acids (aa) in length and contains one fork-head DNA binding domain (aa 120‑210). The presence of basic residues in the fork-head domain makes FoxJ1 a class 1 Fox protein. FoxJ1 induces cilia in lung and oviduct and maintains T cell tolerance to self-antigens. It is known to modulate NF kappa B activity. Over the range of amino acids used for immunization, human FoxJ1 is 89% aa identical to dog FoxJ1 and 87% aa identical to both mouse and rat FoxJ1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FOXJ1
• Uniprot:
  • Q92949.3()