FOXG1 Overexpression Lysate

Western Blot: FOXG1 Overexpression Lysate [NBP2-10480] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FOXG1.

• Reactivity: Hu
• Applications: WB

FOXG1 Overexpression Lysate Summary

• Description:

  FOXG1 Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC207964
  
  Accession#: NM_005249
  
  Protein Tag: C-MYC/DDK
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
• Gene: FOXG1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
  
  Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
• Theoretical MW: 52.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for FOXG1 Overexpression Lysate

• BF-1
• BF1HBF-2
• BF2
• BF-2
• Brain factor 1
• Brain factor 2
• FHKL3
• FKHL1forkhead-like 1
• FKHL2
• FKHL3
• FKHL4
• forkhead box G1
• forkhead box G1A
• forkhead box G1B
• forkhead box G1C
• Forkhead box protein G1A
• Forkhead box protein G1B
• Forkhead box protein G1C
• forkhead-like 3
• forkhead-like 4
• Forkhead-related protein FKHL1
• Forkhead-related protein FKHL2
• Forkhead-related protein FKHL3
• FOXG1A
• FOXG1Bforkhead-like 2
• FOXG1C
• HBF-1
• hBF-2
• HBF-3
• HFK1HBF-G2
• HFK2HBF2
• HFK3KHL2
• oncogene QIN
• QINFKH2forkhead box protein G1

Background

Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon. This gene participates in signal transduction Activin A signaling regulation, TGF-beta receptor signaling pathway, transcription factors in neurogenesis, as well as regulation of nuclear SMAD2/3 signaling. It is thought to interact with genes FOXO1, FOXO3, FOXO4, SMAD2, and SMAD3. FOXG1 is associated with diseases such as melanoma, neuronitis, glioblastoma, purpura, west syndrome, rett syndrome, medulloblastoma, hypotonia, retinitis, maternal uniparental disomy, chromosome 14, intellectual disabilities, brain malformations, multiple sclerosis, and lupus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: FOXG1