Follistatin-like 1/FSTL1 Antibody [Unconjugated]

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Western blot shows lysates of Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Follistatin‑like 1/FSTL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Follistatin-like 1/FSTL1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Follistatin-like 1/FSTL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1694) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Follistatin-like 1/FSTL1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Follistatin-like 1/FSTL1
Met1-Ile308
Accession # Q12841
Specificity
Detects human Follistatin-like 1/FSTL1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross‑reactivity with recombinant mouse FSTL1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FSTL1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF1694 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Follistatin-like 1/FSTL1 Antibody [Unconjugated]

  • Flik
  • FLJ50214
  • Follistatin-like 1
  • Follistatin-like protein 1
  • follistatin-related protein 1
  • Follistatin-related Protein
  • FRP
  • FRPFLJ52277
  • FSL1
  • FSTL1
  • TSC-36

Background

Follistatin-like 1 (FSL1 or FSTL1), also known as FRP (follistatin-related protein), Flik (follistatin-like), and TSC-36 (TGF-beta 1-stimulated clone 36), is a secreted 45‑55 kDa extracellular glycoprotein belonging to the BM-40/SPARC/Osteonectin family (1-3). The human FSTL1 cDNA encodes 308 amino acids (aa), including a 20 aa signal sequence, a cysteine-rich Follistatin (EGF- and kazal-like) domain, two apparently non-functional EF-hand calcium-binding motifs, and a von Willebrand Factor C homology domain (1, 3). Mature human FSTL1 shares 94%, 95%, 98%, and 99% aa identity with mouse, rat, bovine, and equine FSTL1, respectively. FSTL1 was first identified as a TGF-beta-induced protein from a mouse osteoblast cell line (4). It is ubiquitously expressed in early mouse development, but is mainly mesenchymal later in development (5). In humans, FSTL1 is a common rheumatoid arthritis auto-antigen (2). It is reported to be either pro-inflammatory due to promoting inflammatory cytokine secretion, or to prevent autoimmune arthritis by inhibiting matrix metalloproteinase (MMP) and prostaglandin expression (6-9). In muscle and heart, it appears to be protective and promotes endothelial cell functions such as revascularization after ischemia, probably due to promoting expression and activation of the protein kinase AKT1 (10, 11). Cardiac and circulating FSTL1 is generally increased in conditions such as heart failure and acute coronary syndrome (11, 12). FSTL1 also appears to be a tumor suppressor, showing down-regulated expression in many human cancers (4, 14, 15). In vitro, it slows proliferation and MMP-dependent migration, and increases FAS-dependent apoptosis of tumor cell lines (14).

  1. Zwijsen, A. et al. (1994) Eur. J. Biochem. 225:937.
  2. Tanaka, M. et al. (1998) Int. Immunol. 10:1305.
  3. Hambrook, H.O. et al. (2004) J. Biol. Chem. 279:11727.
  4. Shibanuma, M. et al. (1993) Eur. J. Biochem. 217:13.
  5. Adams, D. et al. (2007) Gene Expr. Patterns 7:491.
  6. Clutter, S.D. et al. (2009) J. Immunol. 182:234.
  7. Miyamae, T. et al. (2006) J. Immunol. 177:4758.
  8. Kawabata, D. et al. (2004) Arthritis Rheum. 50:660.
  9. Tanaka, M. et al. (2003) Int. Immunol. 15:71.
  10. Ouchi, N. et al. (2008) J. Biol. Chem. 283:32802.
  11. Oshima, Y. et al. (2008) Circulation 117:3099.
  12. Lara-Pezzi, E. et al. (2008) Endocrinology 149:5822.
  13. Widera, C. et al. (2009) Clin. Chem. 55:1794.
  14. Chan, Q.K. et al. (2009) Carcinogenesis 30:114.
  15. Mashimo, J. et al. (1997) Cancer Lett. 113:213.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Follistatin-like 1/FSTL1 Antibody (AF1694)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ELISA Development (Capture), Western Blot.


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Western Blot
(3)
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Biotin BAF1694

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Bioinformatics

Gene Symbol FSTL1
Uniprot