Follistatin-like 1/FSTL1 Antibody (228208) [Unconjugated]

Follistatin-like 1/FSTL1 was detected in immersion fixed PC-12 rat adrenal pheochromocytoma cell line using Rat Anti-Mouse/Rat Follistatin-like 1/FSTL1 Mono-clonal Antibody (Catalog # MAB17381) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Mu, Rt
• Applications: ICC/IF
• Clone: 228208
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Follistatin-like 1/FSTL1 Antibody (228208) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Follistatin-like 1/FSTL1
  Glu19-Ile306
  Accession # NP_032073
• Specificity: Detects mouse Follistatin-like 1/FSTL1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: FSTL1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Follistatin-like 1/FSTL1 Antibody (228208) [Unconjugated]

• Flik
• FLJ50214
• Follistatin-like 1
• Follistatin-like protein 1
• follistatin-related protein 1
• Follistatin-related Protein
• FRP
• FRPFLJ52277
• FSL1
• FSTL1
• TSC-36

Background

Follistatin-Like 1 (FSTL1), also known as FSTL1 and follistatin-related protein (FRP), is a secreted protein that contains a follistatin-like domain, a Kazal-like domain and a von Willebrand factor C domain. Human FSTL1 shares 92% amino acid sequence identity with the mouse FSTL1, also known as TSC-36.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Follistatin-like 1/FSTL1 Antibody (MAB17381)(2)

Publications using MAB17381

• Y Fang, S Zhang, X Li, F Jiang, Q Ye, W Ning Follistatin like-1 aggravates silica-induced mouse lung injury Sci Rep, 2017-03-24;7(1):399. 2017-03-24 [PMID: 28341862] (IHC, Mouse)
• Wei K, Serpooshan V, Hurtado C, Diez-Cunado M, Zhao M, Maruyama S, Zhu W, Fajardo G, Noseda M, Nakamura K, Tian X, Liu Q, Wang A, Matsuura Y, Bushway P, Cai W, Savchenko A, Mahmoudi M, Schneider M, van den Hoff M, Butte M, Yang P, Walsh K, Zhou B, Bernstein D, Mercola M, Ruiz-Lozano P Epicardial FSTL1 reconstitution regenerates the adult mammalian heart. Nature, 2015-09-16;525(7570):479-85. 2015-09-16 [PMID: 26375005] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: FSTL1
• Uniprot:
  • NP_032073()