FNBP1 Antibody - BSA Free

Western Blot: FNBP1 Antibody [NBP1-47260] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-FBP17 antibody NBP1-47260 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. FBP17 was also immunoprecipitated by rabbit anti-FBP17 antibody NBP1-47261, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

• Reactivity: Hu
• Applications: WB, IP, MA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

FNBP1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit FNBP1 Antibody - BSA Free (NBP1-47260) is a polyclonal antibody validated for use in WB and IP. Anti-FNBP1 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 350 and 400 of human Formin-Binding Protein 17 using the numbering given in entry NP_055848.1 (GeneID 23048).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FNBP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 5-15 ug/mg lysate
  • Microarray
  • Western Blot 1:2000-1:10000
• Application Notes: Use in MiAr reported in secitific publication PMID: 32665637

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FNBP1 Antibody - BSA Free

• FBP17KIAA0554Formin-binding protein 17
• formin binding protein 1
• formin-binding protein 1
• hFBP17
• MGC126804

Background

The protein recognised by this antibody is a member of the formin binding protein family. The protein contains an N terminal Fer/Cdc42 interacting protein 4 (CIP4) homology (FCH) domain followed by a coiled coil domain, a proline rich motif, a second coiled coil domain, a Rho family protein binding domain (RBD), and a C terminal SH3 domain. This protein binds sorting nexin 2 (SNX2), tankyrase (TNKS), and dynamin; an interaction between this protein and formin has not been demonstrated yet in human.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FNBP1 Antibody (NBP1-47260)(3)

Publications using NBP1-47260

• Suman P, Mehta V, Craig AWB, Chander H Wild Type p53 suppresses Formin Binding Protein 17 (FBP17) to reduce invasion Carcinogenesis 2022-02-03 [PMID: 35134126]
• Suman P, Mishra S, Chander H High formin binding protein 17 (FBP17) expression indicates poor differentiation and invasiveness of ductal carcinomas Sci Rep 2020-07-14 [PMID: 32665637] (MiAr, Human)
• Suman P, Mishra S, Chander H. High expression of FBP17 in invasive breast cancer cells promotes invadopodia formation. Med. Oncol. 2018-04-12 [PMID: 29651632] (WB, Human)

Bioinformatics

• Gene Symbol: FNBP1
• Entrez:
  • 23048(Human)