Recombinant Human Flt-3 Ligand/FLT3L Protein

SDS-Page: Human Flt-3 Ligand Protein [NBP2-34868]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human Flt-3 Ligand/FLT3L Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 155 amino acids corresponding to Flt-3 Ligand/FLT3L Source: E. coli

  Uniprot ID: P49771

  Amino Acid Sequence: TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA

• Details of Functionality: Flt3 Ligand protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human AML5 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 x 10^6 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: FLT3LG
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of Flt-3 Ligand/FLT3L as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 17.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.0.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human Flt-3 Ligand/FLT3L Protein

• FL
• FLG3L
• Flt3 ligand
• Flt-3 Ligand
• Flt3L
• FLT3LG
• fms-related tyrosine kinase 3 ligand
• SL cytokine

Background

Flt-3 Ligand promotes the differentiation of multiple hematopoietic cell lineages. Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts. Both transmembrane and soluble forms of Flt-3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2. Flt-3 Ligand induces the expansion of monocytes and immature dendritic cells, B cell differentiation, NK cell development, and myeloid differentiation of hematopoietic stem cells. The mouse and human proteins show cross-species activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: FLT3LG
• Uniprot:
  • P49771(Human)