Flt-3 Ligand/FLT3L Antibodies: Novus Biologicals

AF427

Flt-3 Ligand/FLT3L Antibody [Unconjugated]

100 μg

AF427

427-FL) stimulates proliferation in BaF3 mouse pro-B cell line transfected with mouse Flt-3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Flt-3 Ligand/FLT3L (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Flt-3 Ligand/FLT3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF427). The ND₅₀ is typically 0.05-0.25 µg/mL." class="big_lightbox">

Recombinant Mouse Flt-3 Ligand/FLT3L (Catalog # <a class=

427-FL) stimulates proliferation in BaF3 mouse pro-B cell line transfected with mouse Flt-3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Flt-3 Ligand/FLT3L (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Flt-3 Ligand/FLT3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF427). The ND₅₀ is typically 0.05-0.25 µg/mL." alt="Recombinant Mouse Flt-3 Ligand/FLT3L (Catalog # 427-FL) stimulates proliferation in BaF3 mouse pro-B cell line transfected with mouse Flt-3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Flt-3 Ligand/FLT3L (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Flt-3 Ligand/FLT3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF427). The ND₅₀ is typically 0.05-0.25 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

Flt-3 Ligand/FLT3L was detected in immersion fixed HT-2 mouse T cell line using Goat Anti-Mouse Flt-3 Ligand/FLT3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF427) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

Flt3L-producing tumors do not expand mature ILCs in periphery. Mice were injected with 2 × 106 B16-Flt3L or B16/PBS as a control. Two weeks after tumor injection, ILCs were analyzed by FACS in the lungs, small intestine and colonic lamina propria. (a) Representative dot plots showing NK1.1+T-bet+Eomes− ILC1, NK1.1+T-bet+Eomes+ cNK, GATA-3+ ILC2, ROR gamma t+ ILC3 and NKp46+ and CCR6+ ILC3 subsets gated on CD45+ Lin (CD3, CD19)neg CD90+ cells in the colonic lamina propria and (b) quantification of absolute numbers (n = 5 to 7/group, 3 experiments). Representative dot plots (c) and absolute numbers (d) of ILC2 and ILC3 in the small intestine lamina propria and lungs of B16-Flt3L and B16/PBS injected mice (n = 5 to 8/group, 3 experiments). (e) Representative dot plots of IL22+ ROR gamma t+ ILC3 and IL-5+ GATA-3+ ILC2 in the small intestine of B16-Flt3L and B16 injected mice (n = 3 to 4, 2 experiments). *P < 0.05; ns, not significant; Student’s t-test (b,d,e). Error bars represent SEM in all panels. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29317685), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Flt3L-producing tumors expand CHILPs in the BM. Mice were injected with 2 × 106 B16-Flt3L or B16/PBS as a control. Two weeks after tumor injection, Flt3L serum levels were analyzed by ELISA and CHILPs and ILC2P were analyzed by FACS on BM single-cell suspension. (a) Flt3L serum levels in B16/PBS and B16-Flt3L injected mice (n = 3/group, 2 experiments). Representative dot plots (b) and total number (c) of Lin− alpha 4 beta 7+ cells in the BM of wild-type mice injected with B16-Flt3L or B16/PBS (n = 6/group, 3 experiments). Representative dot plots (d) and absolute numbers (e) of CHILPs and ILC2P in the BM of Id2Gfp/+ mice injected with B16-Flt3L or B16/PBS (n = 6/group, 3 experiments). (f) Correlation plot between Flt3L serum levels determined by ELISA and BM CHILPs absolute numbers determined by FACS in mice treated with B16-Flt3L or B16 (n = 6, 2 experiments). Representative dot plots (g) and absolute numbers (h) of ILCP in the bone marrow of wild type mice injected with B16-Flt3L or B16 cells (n = 5 to 7/group, 3 experiments). *P < 0.05; **P < 0.01; ns, not significant; Student’s t-test (c,e,h), Linear regression with Pearson’s correlation analysis (f). Error bars represent SEM in all panels. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29317685), licensed under a CC-BY license. Not internally tested by R&D Systems.

427-FL) was serially diluted and captured by Goat Anti-Mouse Flt-3 Ligand/FLT3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF427) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Flt-3 Ligand/FLT3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF427) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Mouse Flt‑3 Ligand (Catalog # <a class=

427-FL) was serially diluted and captured by Goat Anti-Mouse Flt-3 Ligand/FLT3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF427) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Flt-3 Ligand/FLT3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF427) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

427-FL) was serially diluted and captured by Goat Anti-Mouse Flt-3 Ligand/FLT3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF427) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Flt-3 Ligand/FLT3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF427) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

427-FL) was serially diluted and captured by Goat Anti-Mouse Flt-3 Ligand/FLT3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF427) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Flt-3 Ligand/FLT3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF427) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF427U-100UG
Alexa Fluor 405	AF427V-100UG
Alexa Fluor 488	AF427G-100UG
Alexa Fluor 532	AF427X-100UG
Alexa Fluor 594	AF427T-100UG
Alexa Fluor 647	AF427R-100UG
Alexa Fluor 700	AF427N-100UG
Alexa Fluor 750	AF427S-100UG
Alexa Fluor Plus 405	AF427AFP405-100UG
Alexa Fluor Plus 488	AF427AFP488-100UG
Alexa Fluor Plus 555	AF427AFP555-100UG
Alexa Fluor Plus 594	AF427AFP594-100UG
Alexa Fluor Plus 647	AF427AFP647-100UG
Alexa Fluor Plus 680	AF427AFP680-100UG
Allophycocyanin	AF427A
Biotin	BAF427
CoraFluor 1	AF427CL1
DyLight 350	AF427D
DyLight 405	AF427E
DyLight 488	AF427K
DyLight 550	AF427L
DyLight 594	AF427M
DyLight 650	AF427W
DyLight 680	AF427Y
DyLight 755	AF427Z
FITC	AF427F
HRP	AF427H
Janelia Fluor 525	AF427JF525
Janelia Fluor 549	AF427I
Janelia Fluor 646	AF427J
Janelia Fluor 669	AF427JF669
PE	AF427P
PerCP	AF427C
mFluor Violet 450 SE	AF427MFV450
mFluor Violet 500 SE	AF427MFV500
mFluor Violet 610 SE	AF427MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF427-SP

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MAB608

Flt-3 Ligand/FLT3L Antibody (40416) [Unconjugated]

(3 Publications)

100 μg

MAB608

308-FK) stimulates proliferation in BaF3 mouse pro-B cell line transfected with mouse Flt-3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Flt-3 Ligand/FLT3L (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Flt-3 Ligand/FLT3L Monoclonal Antibody (Catalog # MAB608). The ND₅₀ is typically 0.02-0.06 µg/mL." class="big_lightbox">

Recombinant Human Flt-3 Ligand/FLT3L (Catalog # <a class=

308-FK) stimulates proliferation in BaF3 mouse pro-B cell line transfected with mouse Flt-3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Flt-3 Ligand/FLT3L (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Flt-3 Ligand/FLT3L Monoclonal Antibody (Catalog # MAB608). The ND₅₀ is typically 0.02-0.06 µg/mL." alt="Recombinant Human Flt-3 Ligand/FLT3L (Catalog # 308-FK) stimulates proliferation in BaF3 mouse pro-B cell line transfected with mouse Flt-3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Flt-3 Ligand/FLT3L (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Flt-3 Ligand/FLT3L Monoclonal Antibody (Catalog # MAB608). The ND₅₀ is typically 0.02-0.06 µg/mL." class="big_thumb" />

308-FKN) was serially diluted and captured by Mouse Anti-Human Flt-3 Ligand/FLT3L Monoclonal Antibody (Catalog # MAB608) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Flt-3 Ligand/FLT3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-308-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Human Flt‑3 Ligand (Catalog # <a class=

308-FKN) was serially diluted and captured by Mouse Anti-Human Flt-3 Ligand/FLT3L Monoclonal Antibody (Catalog # MAB608) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Flt-3 Ligand/FLT3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-308-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB608U-100UG
Alexa Fluor 405	FAB608V-100UG
Alexa Fluor 488	FAB608G-100UG
Alexa Fluor 532	FAB608X-100UG
Alexa Fluor 594	FAB608T-100UG
Alexa Fluor 647	FAB608R-100UG
Alexa Fluor 700	FAB608N-100UG
Alexa Fluor 750	FAB608S-100UG
Alexa Fluor Plus 405	FAB608AFP405-100UG
Alexa Fluor Plus 488	FAB608AFP488-100UG
Alexa Fluor Plus 555	FAB608AFP555-100UG
Alexa Fluor Plus 594	FAB608AFP594-100UG
Alexa Fluor Plus 647	FAB608AFP647-100UG
Alexa Fluor Plus 680	FAB608AFP680-100UG
Allophycocyanin	FAB608A
Biotin	FAB608B
CoraFluor 1	FAB608CL1
DyLight 350	FAB608D
DyLight 405	FAB608E
DyLight 488	FAB608K
DyLight 550	FAB608L
DyLight 594	FAB608M
DyLight 650	FAB608W
DyLight 680	FAB608Y
DyLight 755	FAB608Z
FITC	FAB608F
HRP	FAB608H
Janelia Fluor 525	FAB608JF525
Janelia Fluor 549	FAB608I
Janelia Fluor 646	FAB608J
Janelia Fluor 669	FAB608JF669
PE	FAB608P
PerCP	FAB608C
mFluor Violet 450 SE	FAB608MFV450
mFluor Violet 500 SE	FAB608MFV500
mFluor Violet 610 SE	FAB608MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB608-SP
Carrier Free	MAB608-500

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MAB308

Flt-3 Ligand/FLT3L Antibody (40406) [Unconjugated]

(4 Publications)

100 μg

MAB308

Recombinant Human Flt-3 Ligand/FLT3L (Catalog # 308-FK or 308-FKN) stimulates proliferation in the BaF3 mouse pro-B cell line transfected with mouse Flt-3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Flt-3 Ligand/FLT3L (5 ng/mL) is neutralized (green line) by increasing concentrations of Human Flt-3 Ligand/FLT3L Monoclonal Antibody (Catalog # MAB308). The ND<SUB>50</SUB> is typically 0.02-0.06 µg/mL.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB308U-100UG
Alexa Fluor 405	FAB308V-100UG
Alexa Fluor 488	FAB308G-100UG
Alexa Fluor 532	FAB308X-100UG
Alexa Fluor 594	FAB308T-100UG
Alexa Fluor 647	FAB308R-100UG
Alexa Fluor 700	FAB308N-100UG
Alexa Fluor 750	FAB308S-100UG
Alexa Fluor Plus 405	FAB308AFP405-100UG
Alexa Fluor Plus 488	FAB308AFP488-100UG
Alexa Fluor Plus 555	FAB308AFP555-100UG
Alexa Fluor Plus 594	FAB308AFP594-100UG
Alexa Fluor Plus 647	FAB308AFP647-100UG
Alexa Fluor Plus 680	FAB308AFP680-100UG
Allophycocyanin	FAB308A
Biotin	FAB308B
CoraFluor 1	FAB308CL1
DyLight 350	FAB308D
DyLight 405	FAB308E
DyLight 488	FAB308K
DyLight 550	FAB308L
DyLight 594	FAB308M
DyLight 650	FAB308W
DyLight 680	FAB308Y
DyLight 755	FAB308Z
FITC	FAB308F
HRP	FAB308H
Janelia Fluor 525	FAB308JF525
Janelia Fluor 549	FAB308I
Janelia Fluor 646	FAB308J
Janelia Fluor 669	FAB308JF669
PE	FAB308P
PerCP	FAB308C
mFluor Violet 450 SE	FAB308MFV450
mFluor Violet 500 SE	FAB308MFV500
mFluor Violet 610 SE	FAB308MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB308-SP
Carrier Free	MAB308-500

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AF-308-NA

Flt-3 Ligand/FLT3L Antibody [Unconjugated]

(2 Publications)

100 μg

AF-308-NA

308-FK) stimulates proliferation in BaFlt3 mouse pro-B cell line transfected with mouse Flt-3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Flt-3 Ligand/FLT3L (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Flt-3 Ligand/FLT3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-308-NA). The ND₅₀ is typically 20‑250 ng/mL." class="big_lightbox">

308-FK) stimulates proliferation in BaFlt3 mouse pro-B cell line transfected with mouse Flt-3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Flt-3 Ligand/FLT3L (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Flt-3 Ligand/FLT3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-308-NA). The ND₅₀ is typically 20‑250 ng/mL." alt="Recombinant Human Flt-3 Ligand/FLT3L (Catalog # 308-FK) stimulates proliferation in BaFlt3 mouse pro-B cell line transfected with mouse Flt-3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Flt-3 Ligand/FLT3L (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Flt-3 Ligand/FLT3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-308-NA). The ND₅₀ is typically 20‑250 ng/mL." class="big_thumb" />

308-FKN) was serially diluted and captured by Mouse Anti-Human Flt-3 Ligand/FLT3L Monoclonal Antibody (Catalog # MAB608) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Flt-3 Ligand/FLT3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-308-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

308-FKN) was serially diluted and captured by Mouse Anti-Human Flt-3 Ligand/FLT3L Monoclonal Antibody (Catalog # MAB608) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Flt-3 Ligand/FLT3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-308-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF-308-NAU-100UG
Alexa Fluor 405	AF-308-NAV-100UG
Alexa Fluor 488	AF-308-NAG-100UG
Alexa Fluor 532	AF-308-NAX-100UG
Alexa Fluor 594	AF-308-NAT-100UG
Alexa Fluor 647	AF-308-NAR-100UG
Alexa Fluor 700	AF-308-NAN-100UG
Alexa Fluor 750	AF-308-NAS-100UG
Alexa Fluor Plus 405	AF-308-NAAFP405
Alexa Fluor Plus 488	AF-308-NAAFP488
Alexa Fluor Plus 555	AF-308-NAAFP555
Alexa Fluor Plus 594	AF-308-NAAFP594
Alexa Fluor Plus 647	AF-308-NAAFP647
Alexa Fluor Plus 680	AF-308-NAAFP680
Allophycocyanin	AF-308-NAA
Biotin	BAF308
CoraFluor 1	AF-308-NACL1
DyLight 350	AF-308-NAD
DyLight 405	AF-308-NAE
DyLight 488	AF-308-NAK
DyLight 550	AF-308-NAL
DyLight 594	AF-308-NAM
DyLight 650	AF-308-NAW
DyLight 680	AF-308-NAY
DyLight 755	AF-308-NAZ
FITC	AF-308-NAF
HRP	AF-308-NAH
Janelia Fluor 525	AF-308-NAJF525
Janelia Fluor 549	AF-308-NAI
Janelia Fluor 646	AF-308-NAJ
Janelia Fluor 669	AF-308-NAJF669
PE	AF-308-NAP
PerCP	AF-308-NAC
mFluor Violet 450 SE	AF-308-NAMFV450
mFluor Violet 500 SE	AF-308-NAMFV500
mFluor Violet 610 SE	AF-308-NAMFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF-308-SP

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AF2467

Flt-3 Ligand/FLT3L Antibody [Unconjugated]

100 μg

AF2467

2467-FL) stimulates proliferation in BaFlt3 mouse pro-B cell line transfected with mouse Flt-3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline Flt-3 Ligand/FLT3L (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Feline Flt-3 Ligand/FLT3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2467). The ND₅₀ is typically 0.1-0.4 µg/mL." class="big_lightbox">

Recombinant Feline Flt-3 Ligand/FLT3L (Catalog # <a class=

2467-FL) stimulates proliferation in BaFlt3 mouse pro-B cell line transfected with mouse Flt-3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline Flt-3 Ligand/FLT3L (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Feline Flt-3 Ligand/FLT3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2467). The ND₅₀ is typically 0.1-0.4 µg/mL." alt="Recombinant Feline Flt-3 Ligand/FLT3L (Catalog # 2467-FL) stimulates proliferation in BaFlt3 mouse pro-B cell line transfected with mouse Flt-3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline Flt-3 Ligand/FLT3L (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Feline Flt-3 Ligand/FLT3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2467). The ND₅₀ is typically 0.1-0.4 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

Flt-3 Ligand/FLT3L was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) using Goat Anti-Feline Flt-3 Ligand/FLT3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2467) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

Reactivity:

Fe

Species Glossary

Reactivity: Fe

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2467U-100UG
Alexa Fluor 405	AF2467V-100UG
Alexa Fluor 488	AF2467G-100UG
Alexa Fluor 532	AF2467X-100UG
Alexa Fluor 594	AF2467T-100UG
Alexa Fluor 647	AF2467R-100UG
Alexa Fluor 700	AF2467N-100UG
Alexa Fluor 750	AF2467S-100UG
Alexa Fluor Plus 405	AF2467AFP405-100UG
Alexa Fluor Plus 488	AF2467AFP488-100UG
Alexa Fluor Plus 555	AF2467AFP555-100UG
Alexa Fluor Plus 594	AF2467AFP594-100UG
Alexa Fluor Plus 647	AF2467AFP647-100UG
Alexa Fluor Plus 680	AF2467AFP680-100UG
Allophycocyanin	AF2467A
Biotin	AF2467B
CoraFluor 1	AF2467CL1
DyLight 350	AF2467D
DyLight 405	AF2467E
DyLight 488	AF2467K
DyLight 550	AF2467L
DyLight 594	AF2467M
DyLight 650	AF2467W
DyLight 680	AF2467Y
DyLight 755	AF2467Z
FITC	AF2467F
HRP	AF2467H
Janelia Fluor 525	AF2467JF525
Janelia Fluor 549	AF2467I
Janelia Fluor 646	AF2467J
Janelia Fluor 669	AF2467JF669
PE	AF2467P
PerCP	AF2467C
mFluor Violet 450 SE	AF2467MFV450
mFluor Violet 500 SE	AF2467MFV500
mFluor Violet 610 SE	AF2467MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2467-SP

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MAB308R

Flt-3 Ligand/FLT3L Antibody (40406R) [Unconjugated]

100 μg

MAB308R

Recombinant Human Flt-3 Ligand (308-FK or 308-FKN) stimulates proliferation in the BaF3 mouse pro-B cell line transfected with mouse Flt-3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Flt-3 Ligand (5 ng/mL) is neutralized (green line) by increasing concentrations of Human Flt-3 Ligand Monoclonal Antibody (Catalog # MAB308R). The ND<SUB>50</SUB> is typically 0.02-0.06 µg/mL.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB308RU-100UG
Alexa Fluor 405	FAB308RV-100UG
Alexa Fluor 488	FAB308RG-100UG
Alexa Fluor 532	FAB308RX-100UG
Alexa Fluor 594	FAB308RT-100UG
Alexa Fluor 647	FAB308RR-100UG
Alexa Fluor 700	FAB308RN-100UG
Alexa Fluor 750	FAB308RS-100UG
Alexa Fluor Plus 405	FAB308RAFP405-100UG
Alexa Fluor Plus 488	FAB308RAFP488-100UG
Alexa Fluor Plus 555	FAB308RAFP555-100UG
Alexa Fluor Plus 594	FAB308RAFP594-100UG
Alexa Fluor Plus 647	FAB308RAFP647-100UG
Alexa Fluor Plus 680	FAB308RAFP680-100UG
Allophycocyanin	FAB308RA
Biotin	FAB308RB
CoraFluor 1	FAB308RCL1
DyLight 350	FAB308RD
DyLight 405	FAB308RE
DyLight 488	FAB308RK
DyLight 550	FAB308RL
DyLight 594	FAB308RM
DyLight 650	FAB308RW
DyLight 680	FAB308RY
DyLight 755	FAB308RZ
FITC	FAB308RF
HRP	FAB308RH
Janelia Fluor 525	FAB308RJF525
Janelia Fluor 549	FAB308RI
Janelia Fluor 646	FAB308RJ
Janelia Fluor 669	FAB308RJF669
PE	FAB308RP
PerCP	FAB308RC
mFluor Violet 450 SE	FAB308RMFV450
mFluor Violet 500 SE	FAB308RMFV500
mFluor Violet 610 SE	FAB308RMFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB308R-SP

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NBP3-41075

Flt-3 Ligand/FLT3L Antibody (D13) - BSA Free

100 ul

NBP3-41075

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00002323-M07

Flt-3 Ligand/FLT3L Antibody (4C4) - Azide and BSA Free

0.1 mg

H00002323-M07

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP1-62304

Flt-3 Ligand/FLT3L Antibody - BSA Free

100 ul

NBP1-62304

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP3-48329

Flt-3 Ligand/FLT3L Antibody (DMC421) - Chimeric

100 μg

NBP3-48329

Flow cytometry analysis with Anti-Flt-3 Ligand/FLT3L (DMC421) on HEK293 cells transfected with human Flt-3 Ligand/FLT3L (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP3-41067

Flt-3 Ligand/FLT3L Antibody (D4) - BSA Free

100 ul

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP2-89408

Flt-3 Ligand/FLT3L Antibody (008) - Azide and BSA Free

100 ul

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-89408AF350
Alexa Fluor 405	NBP2-89408AF405
Alexa Fluor 488	NBP2-89408AF488
Alexa Fluor 532	NBP2-89408AF532
Alexa Fluor 594	NBP2-89408AF594
Alexa Fluor 647	NBP2-89408AF647
Alexa Fluor 700	NBP2-89408AF700
Alexa Fluor 750	NBP2-89408AF750
Alexa Fluor Plus 488	NBP2-89408AFP488
Alexa Fluor Plus 555	NBP2-89408AFP555
Alexa Fluor Plus 594	NBP2-89408AFP594
Alexa Fluor Plus 647	NBP2-89408AFP647
Alexa Fluor Plus 680	NBP2-89408AFP680
Allophycocyanin	NBP2-89408APC
Biotin	NBP2-89408B
CoraFluor 1	NBP2-89408CL1
DyLight 350	NBP2-89408UV
DyLight 405	NBP2-89408V
DyLight 488	NBP2-89408G
DyLight 550	NBP2-89408R
DyLight 594	NBP2-89408DL594
DyLight 650	NBP2-89408C
DyLight 680	NBP2-89408FR
DyLight 755	NBP2-89408IR
FITC	NBP2-89408F
HRP	NBP2-89408H
Janelia Fluor 525	NBP2-89408JF525
Janelia Fluor 549	NBP2-89408JF549
Janelia Fluor 646	NBP2-89408JF646
Janelia Fluor 669	NBP2-89408JF669
PE	NBP2-89408PE
PerCP	NBP2-89408PCP
mFluor Violet 450 SE	NBP2-89408MFV450
mFluor Violet 500 SE	NBP2-89408MFV500
mFluor Violet 610 SE	NBP2-89408MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-89408-50ul

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Flt-3 Ligand/FLT3L Antibody [Unconjugated]

Flt-3 Ligand/FLT3L Antibody (40416) [Unconjugated]

Flt-3 Ligand/FLT3L Antibody (40406) [Unconjugated]

Flt-3 Ligand/FLT3L Antibody [Unconjugated]

Flt-3 Ligand/FLT3L Antibody [Unconjugated]

Flt-3 Ligand/FLT3L Antibody (40406R) [Unconjugated]

Flt-3 Ligand/FLT3L Antibody (D13) - BSA Free

Flt-3 Ligand/FLT3L Antibody (4C4) - Azide and BSA Free

Flt-3 Ligand/FLT3L Antibody - BSA Free

Flt-3 Ligand/FLT3L Antibody (DMC421) - Chimeric

Flt-3 Ligand/FLT3L Antibody (D4) - BSA Free

Flt-3 Ligand/FLT3L Antibody (008) - Azide and BSA Free