Flotillin-1 Antibody

Images

 
Western Blot: Flotillin-1 Antibody [NB100-1043] - HEK293 overexpressing FLOT1 and probed with (mock transfection in first lane).
Western Blot: Flotillin-1 Antibody [NB100-1043] - Analysis of H460 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. (0.3ug/ml)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Flotillin-1 Antibody Summary

Immunogen
Peptide with sequence C-SISQVNHKPLRTA corresponding to C-Terminus according to NP_005794.1.
Localization
Cell Membrane
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FLOT1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Immunohistochemistry 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 48 kDa band observed in lysates of H460 (calculated MW of 47.4 kDa band according to NP_005794.1). In transfected HEK293 transiently expressing FLOT1 a band of approx. 48 kDa is observed. This band is not observed in the non-transfected HEK293. IHC-P: Human kidney shows membrane staning in DCT.
Positive Control
Flotillin-1 Lysate (NBL1-10769)
Publications
Read Publications using
NB100-1043 in the following applications:

  • WB
    1 publication

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Flotillin-1 Antibody

  • flotillin 1
  • flotillin-1
  • integral membrane component of caveolae

Background

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Flotillin-1 Antibody (NB100-1043)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FLOT1
Entrez
Uniprot