Western Blot: Flotillin-1 Antibody [NB100-1043] - HEK293 overexpressing FLOT1 and probed with (mock transfection in first lane).
Western Blot: Flotillin-1 Antibody [NB100-1043] - Analysis of H460 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. (0.3ug/ml)
WB: Approx. 48 kDa band observed in lysates of H460 (calculated MW of 47.4 kDa band according to NP_005794.1). In transfected HEK293 transiently expressing FLOT1 a band of approx. 48 kDa is observed. This band is not observed in the non-transfected HEK293. IHC-P: Human kidney shows membrane staning in DCT.
Predicted cross-reactivity based on sequence identity: Canine, Porcine, Bovine.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for Flotillin-1 Antibody
integral membrane component of caveolae
Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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