Flotillin-1 Antibody

Western Blot: Flotillin-1 Antibody [NB100-1043] - Staining of K562 lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by Chemiluminescence.

Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NB100-1043] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NB100-1043] - Staining of paraffin embedded Human Kidney. Antibody at 5 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu, Po, Bv, Ca
• Applications: WB, Simple Western, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Flotillin-1 Antibody Summary

• Immunogen: Peptide with sequence C-SISQVNHKPLRTA corresponding to C-Terminus according to NP_005794.1.
• Localization: Cell Membrane
• Predicted Species: Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FLOT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Simple Western
  • Western Blot 0.1 - 0.3 ug/mL
• Application Notes: Western blot: Approx 48 kDa band observed in lysates of cell line K562 and in preliminary testing of cell lysate MCF7 (calculated MW of 47.4 kDa according to NP_005794.1).
  See Simple Western Antibody Database for Simple Western validation: Tested in Brain, separated by Size
• Control:

  Flotillin-1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Flotillin-1 Antibody

• flotillin 1
• flotillin-1
• integral membrane component of caveolae

Background

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Flotillin-1 Antibody (NB100-1043)(4)

Publications using NB100-1043

• Pushkarsky T, Ward A, Ivanov A et al. Abundance of Nef and p-Tau217 in Brains of Individuals Diagnosed with HIV-Associated Neurocognitive Disorders Correlate with Disease Severance Molecular Neurobiology 2022-02-01 [PMID: 34843091]
• Pedro Fuentes, Marta Sesé, Pedro J. Guijarro, Marta Emperador, Sara Sánchez-Redondo, Héctor Peinado, Stefan Hümmer, Santiago Ramón y. Cajal ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells Nature Communications 2020-08-26 [PMID: 32848136]
• Vassilieva EV, Gerner-Smidt K, Ivanov AI, Nusrat A. Lipid rafts mediate internalization of beta1-integrin in migrating intestinal epithelial cells. Am J Physiol Gastrointest Liver Physiol. 2008-11-01 [PMID: 18755811] (WB, Human)
  
  Details:
  WB (Human SK-CO15 cells), Fig. 10A.
• Bickel PE, Scherer PE, Schnitzer JE et al. Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins. J Biol Chem 1997-05-23 [PMID: 9153235]

Bioinformatics

• Gene Symbol: FLOT1
• Entrez:
  • 10211(Human)
  • 14251(Mouse)
• Uniprot:
  • NP_005794.(Human)