FLI1 Antibody (FLI1/1312)

Product Discontinued

FLI1 Antibody (FLI1/1312) Summary

• Immunogen: Recombinant fragment (aa 107-261) of human FLI1 protein with hexa-histidine tag
• Localization: Nuclear
• Marker: Ewing’s Sarcoma & Endothelial Marker
• Specificity: Recognizes a protein of 51kDa, which is identified as FLI1. This protein, a member of the ETS family of DNA binding transcription factors, is involved in cellular proliferation and tumorigenesis. Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. Members of the Ets gene family share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. Approximately 90% of Ewing's Sarcoma (EWS) / Primitive Neuroectodermal Tumors (PNET) have a specific translocation, t(11;22)(q24;q12), which results in fusion of EWS to Fli-1, and production of an EWS-Fli-1 fusion protein. Among normal tissues only endothelial cells and small lymphocytes express Fli-1. This protein is expressed in majority of vascular tumors including angiosarcomas, hemangioendotheliomas, hemangiomas, and Kaposi's Sarcomas. High sensitivity and specificity of Fli-1 equals to or exceeds that of the established vascular markers like CD31, CD34, and Factor VIII.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FLI1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5-1ug/million cells
  • Immunofluorescence 1-2ug/ml
  • Western Blot 1-2ug/ml
• Application Notes: Human Chromosome Location: 11q24.1-q24.3
  Western Blotting (1-2ug/ml for 60 minutes at RT)
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10mM PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Alternate Names for FLI1 Antibody (FLI1/1312)

• ERGB
• EWSR2
• FLI1
• Friend leukemia integration 1 transcription factor
• Friend leukemia virus integration 1
• Proto-oncogene Fli-1
• SIC-1
• Transcription factor ERGB

Background

Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2 Erg-1, Erg-2, Elk, E74, Fli-1, PU.1 and PEA3. Members of the Ets gene family exhibit varied pattern of tissue expression, and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FLI1