FKBP51/FKBP5 Antibody

Western Blot: FKBP51/FKBP5 Antibody [NB100-68240] - Normalized expression of TGF-b mRNA in WT, EV-, or FKBP51/FKBP5-stably transfected melanoma cells. WT sample expression 1/4 1. (n 1/4). Western blot assay of TGF-b levels in the same cells. Anti-Flag-labelled exogenous FKBP51/FKBP5. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2013109), licensed under a CC-BY license.

Immunohistochemistry: FKBP51/FKBP5 Antibody [NB100-68240] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-FKBP5/FKBP51 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

Western Blot: FKBP51/FKBP5 Antibody [NB100-68240] - Western Blot and Immunoprecipitation: FKBP5/FKBP51 Antibody NB100-68240 - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-68240 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. FKBP5/FKBP51 was also immunoprecipitated by rabbit anti-FKBP5/FKBP51 antibody NB100-68239, which recognizes an upstream epitope.

Western Blot: FKBP51/FKBP5 Antibody [NB100-68240] - FKBP51/FKBP5 modulates EMT hallmarks. FKBP51/FKBP5 silencing of SAN melanoma cells decreases TGF-b and TbRIII expression. Normalized expression rates (mean +/-S.D.) of TGF-b mRNA levels; (n 1/4). FKBP51/FKBP5 siRNA-treated sample expression 1/4. 1. Western blot assay of TGF-b in SAN melanoma cells treated with nonsilencing (NS) RNA or FKBP51/FKBP5 siRNA. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2013109), licensed under a CC-BY license./4

Western Blot: FKBP51/FKBP5 Antibody [NB100-68240] - FKBP51 increases expression of melanoma CSC markers. (a) FKBP51 silencing decreases ABCB5 levels. (Left) Normalized expression rates (arbitrary units (AU)) (mean±S.D.) of FKBP51 (black) & ABCB5 mRNA (grey) (n=3). FKBP51-treated sample expression=1. (Right) Western blot assay of ABCB5 & FKBP51 levels. (b) FKBP51 overexpression enhances ABCB5 levels. (Left) Normalized expression of FKBP51 mRNA (black) & ABCB5 mRNA (grey) measured in WT, EV-, or FKBP51-stably transfected melanoma cells. WT sample expression=1; n=3. (Left) Western blot assay of ABCB5 & FKBP51 levels in the same cells. Anti-Flag-labelled exogenous FKBP51. (c) ABCG2+ melanoma cells increase in FKBP51-overexpressing cells. (Upper) Normalized expression of FKBP51 mRNA (black) & ABCG2 mRNA (grey) measured in WT, EV-, or FKBP51-stably transfected melanoma cells. WT sample expression=1; n=5. (Lower) Flow cytometric histograms of ABCG2 expression (green population); mean±S.D. of counts are shown. (d) Enhanced FKBP51 mRNA levels in sorted ABCG2+ melanoma cells (SAN, upper; A375, lower). Whole cell expression=1; (n=3). (e) Enhanced EMT gene transcripts in sorted ABCG2+ melanoma cells. ABCG2− sample expression=1; n=3. (f) Expression of ABCG2 transcript in 9 deparaffinized tumours, 4 primary melanoma (M) samples, & 5 metastases (MM). A naevus sample was arbitrarily chosen with expression=1 Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2013109), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: FKBP51/FKBP5 Antibody [NB100-68240] - FKBP51 increases expression of melanoma CSC markers. (a) FKBP51 silencing decreases ABCB5 levels. (Left) Normalized expression rates (arbitrary units (AU)) (mean±S.D.) of FKBP51 (black) & ABCB5 mRNA (grey) (n=3). FKBP51-treated sample expression=1. (Right) Western blot assay of ABCB5 & FKBP51 levels. (b) FKBP51 overexpression enhances ABCB5 levels. (Left) Normalized expression of FKBP51 mRNA (black) & ABCB5 mRNA (grey) measured in WT, EV-, or FKBP51-stably transfected melanoma cells. WT sample expression=1; n=3. (Left) Western blot assay of ABCB5 & FKBP51 levels in the same cells. Anti-Flag-labelled exogenous FKBP51. (c) ABCG2+ melanoma cells increase in FKBP51-overexpressing cells. (Upper) Normalized expression of FKBP51 mRNA (black) & ABCG2 mRNA (grey) measured in WT, EV-, or FKBP51-stably transfected melanoma cells. WT sample expression=1; n=5. (Lower) Flow cytometric histograms of ABCG2 expression (green population); mean±S.D. of counts are shown. (d) Enhanced FKBP51 mRNA levels in sorted ABCG2+ melanoma cells (SAN, upper; A375, lower). Whole cell expression=1; (n=3). (e) Enhanced EMT gene transcripts in sorted ABCG2+ melanoma cells. ABCG2− sample expression=1; n=3. (f) Expression of ABCG2 transcript in 9 deparaffinized tumours, 4 primary melanoma (M) samples, & 5 metastases (MM). A naevus sample was arbitrarily chosen with expression=1 Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2013109), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: FKBP51/FKBP5 Antibody [NB100-68240] - FKBP51 interacts with the general transcriptional co-activator p300. (a, left) FKBP51 co-immunoprecipitates with p300. (a, right), p300 co-immunoprecipitates with FKBP51. Total-cell lysates were prepared by SAN melanoma cells transfected with FKBP51/Flag. Cell lysates were immunoprecipitated with anti-Kat3B/p300 (IP p300) or anti-Flag (IP FKBP51). Immunoprecipitated & total lysates were then subjected to western blot with anti-FKBP51 or anti-p300. (b) ChIP performed with SAN melanoma cells, silenced (FKBP51 siRNA) or not (NS RNA) for FKBP51. An enrichment of DNA (region at −3450 from TSS of ABCG2 gene) can be observed in p300-immunoprecipitated chromatin (NS RNA) compared with IgG sample. Such an enrichment appeared to be reduced when FKBP51 was silenced (FKBP51 siRNA) Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2013109), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Pm
• Applications: WB, ICC/IF, IHC, IP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

FKBP51/FKBP5 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 407 and 457 of human FK506 binding protein 5 (51 kDa FK506-binding protein) using the numbering given in entry NP_004108.1 (GeneID 2289).
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FKBP5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 to 1:1000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Knockdown Validated
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID:28978117).
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  FKBP51/FKBP5 Overexpression Lysate

Reactivity Notes

Orangutan (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FKBP51/FKBP5 Antibody

• 51 kDa FKBP
• 54 kDa progesterone receptor-associated immunophilin
• AIG6,51 kDa FK506-binding protein
• Androgen-regulated protein 6
• Dit1
• FF1 antigen
• FK506 binding protein 5
• FK506-binding protein 5
• FKBP5
• FKBP-5
• FKBP51
• FKBP-51
• FKBP51PPIase FKBP5
• FKBP54
• FKBP54EC 5.2.1.8
• HSP90-binding immunophilin
• MGC111006
• p54
• P54,51 kDa FK506-binding protein 5
• peptidyl-prolyl cis-trans isomerase FKBP5
• peptidylprolyl cis-trans isomerase
• PPIase
• Ptg-10
• rotamase
• T-cell FK506-binding protein

Background

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It is thought to mediate calcineurin inhibition. It also interacts functionally with mature hetero-oligomeric progesterone receptor complexes along with the 90 kDa heat shock protein and P23 protein. This gene has been found to have multiple polyadenylation sites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FKBP51/FKBP5 Antibody (NB100-68240)(7)

Publications using NB100-68240

• Tufano M, Marrone L, D'Ambrosio C et al. FKBP51 plays an essential role in Akt ubiquitination that requires Hsp90 and PHLPP Cell death & disease 2023-02-13 [PMID: 36781840] (WB)
• Tufano M, Cesaro E, Martinelli R et al. FKBP51 Affects TNF-Related Apoptosis Inducing Ligand Response in Melanoma Frontiers in Cell and Developmental Biology 2021-09-13 [PMID: 34589486] (Western Blot, Human)
• Boonying W, Joselin A, Huang E, Qu D et Al. Pink1 regulates FKBP5 interaction with AKT/PHLPP and protects neurons from neurotoxin stress induced by MPP() J Neurochem 2019-02-09 [PMID: 30734931]
• D'arrigo Paolo, Russo Michele, Rea Anna et al. A regulatory role for the co-chaperone FKBP51s in PD-L1 expression in glioma. Oncotarget 2017-07-17 [PMID: 28978117] (KD, WB, ICC/IF, IP, Human)
• Romano S, Xiao Y, Nakaya M et al. FKBP51 employs both scaffold and isomerase functions to promote NF-kB activation in melanoma. Nucleic Acids Res. 2015-06-22 [PMID: 26101251] (IP, WB, Human)
• Romano S, D'Angelillo A, Staibano S et al. Immunomodulatory pathways regulate expression of a spliced FKBP51 isoform in lymphocytes of melanoma patients Pigment Cell Melanoma Res 2015-04-20 [PMID: 25895097] (WB, Human)
• Romano S, Staibano S, Greco A et al. FK506 binding protein 51 positively regulates melanoma stemness and metastatic potential. Cell Death Dis. 2013-04-04 [PMID: 23559012] (WB, Human)

Bioinformatics

• Gene Symbol: FKBP5
• Entrez:
  • 2289(Human)
• Uniprot:
  • Q13451()