Human Fibronectin ELISA Kit (Colorimetric)

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ELISA: Fibronectin/Anastellin ELISA Kit [NBP1-83737] - Standard curve

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, heparin), urine
Standard Curve Range
0.31 - 20.0 ng/mL
Sensitivity
0.1 ng/mL

Order Details

Human Fibronectin ELISA Kit (Colorimetric) Summary

Standard Curve Range
0.31 - 20.0 ng/mL
Sensitivity
0.1 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
6.7%
Intra-Assay
5.3%
Spike Recovery
110.6%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
FN1

Applications/Dilutions

Application Notes
The human Fibronectin ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Fibronectin

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Stop Solution (1M Phosphoric acid)
  2. Substrate Solution (tetramethyl-benzidine)
  3. Streptavidin-HRP
  4. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  5. Human Fibronectin Standard lyophilized, 40 ng/ml upon reconstitution
  6. Biotin-Conjugate anti-human Fibronectin polyclonal antibody
  7. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  8. Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to human Fibronectin
  9. Adhesive Films

Alternate Names for Human Fibronectin ELISA Kit (Colorimetric)

  • Fibronectin
  • FN1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for Fibronectin ELISA Kit (NBP1-83737). (Showing 1 - 1 of 1 FAQ).

  1. I would like to know what was used for the Fibronectin Standard. A recombinant protein, or a human native protein?
    • NBP1-83737 contains a human fibronectin protein standard, which was purified from plasma.

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Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

Fibronectin: Organizing Cell Activity across the ECM
Fibronectin is a glycoprotein found in the extracellular matrix (ECM) that binds to integrins and other components of the ECM such as collagen and fibrin. Under normal physiological conditions, fibronectin is an important factor in cell adhesion, grow...  Read full blog post.

Using the Laminin Antibody in Angiogenesis Research
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Bioinformatics

Gene Symbol FN1
Entrez