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Fibronectin Antibody (2755-8)

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Immunohistochemistry-Paraffin: Fibronectin Antibody (2755-8) [NBP2-32851] - Formalin-fixed, paraffin-embedded human Pancreatic Adeno Carcinoma stained with Fibronectin Monoclonal Antibody (2755-8).
Immunohistochemistry-Paraffin: Fibronectin Antibody (2755-8) [NBP2-32851] - Formalin-fixed, paraffin-embedded human Pancreatic Adeno Carcinoma stained with Fibronectin Monoclonal Antibody (2755-8).

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP
Clone
2755-8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-34526AF350
Alexa Fluor 405
NBP2-34526AF405
Alexa Fluor 488
NBP2-34526AF488
Alexa Fluor 532
NBP2-34526AF532
Alexa Fluor 594
NBP2-34526AF594
Alexa Fluor 647
NBP2-34526AF647
Alexa Fluor 700
NBP2-34526AF700
Alexa Fluor 750
NBP2-34526AF750
Allophycocyanin
NBP2-34526APC
Allophycocyanin/Cy7
NBP2-34526APCCY7
Biotin
NBP2-34526B
DyLight 350
NBP2-34526UV
DyLight 405
NBP2-34526V
DyLight 488
NBP2-34526G
DyLight 550
NBP2-34526R
DyLight 594
NBP2-34526DL594
DyLight 650
NBP2-34526C
DyLight 680
NBP2-34526FR
DyLight 755
NBP2-34526IR
FITC
NBP2-34526F
HRP
NBP2-34526H
Janelia Fluor 549
NBP2-34526JF549
Janelia Fluor 646
NBP2-34526JF646
PE
NBP2-34526PE
PE/Atto594
NBP2-34526PEATT594
PE/Cy5.5
NBP2-34526PECY55
PE/Cy7
NBP2-34526PECY7
PerCP
NBP2-34526PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-34526-0.1 mg
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Fibronectin Antibody (2755-8) Summary

Immunogen
T-cell lymphoma biopsy
Localization
Connective tissue matrix
Specificity
Fibronectin is an extracellular matrix glycoprotein present on most cell surfaces, in extracellular fluids and in plasma. A high molecular weight heterodimeric protein, it was originally discovered as a protein missing from the surfaces of virus-transformed cells, and it has been shown to be involved in various functions including cell adhesion, cell motility and wound healing. Alternative splicing and glycosylation give rise to several different forms of Fibronectin, some of which exhibit restricted tissue distribution or association with malignancies. It has been shown that Myofibroblasts phenotype formation correlates with the occurrence of glycosylated Fibronectin and Fibronectin splice variants in Dupuytren's disease
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FN1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
  • Immunoprecipitation 0.5-1ug/500ug protein lysate
Application Notes
ELISA (For coating, order NBP2-34526 (BSA free version of NBP2-32851))
Control
Fibronectin Knockout HeLa Cell Lysate
Publications
Read Publication using
NBP2-32851 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
PBS with 0.05% BSA.
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A purified

Alternate Names for Fibronectin Antibody (2755-8)

  • CIG
  • ED-B
  • fibronectin 1
  • Fibronectin
  • FINC
  • FN
  • FN1
  • FNZ
  • GFND
  • GFND2
  • LETS
  • MSF
  • SMDCF

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: Vitronectin Antibody (ST49-02) [NBP2-67511] - Staining Vitronectin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunocytochemistry/Immunofluorescence: FAK Antibody (SR46-04) [NBP2-67327] - Staining FAK in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded huamn liver cancer tissue using anti-FAK antibody. Counter stained with hematoxylin.
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Flow (Intracellular): Tenascin C Antibody (4C8MS) [NB110-68136] - Figure A: Intracellular stain performed on U87MG Cells with Tenascin C (4C8MS) antibody NB110-68136 (blue) and a matched isotype control NBP1-97005 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].Figure B: U87MG Cells were either untreated (orange) or treated with 3uM Monensin (blue). An intracellular stain was performed with Tenascin C (4C8MS) antibody NB110-68136. Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].
Immunocytochemistry/Immunofluorescence: Tenascin C Antibody (4C8MS) [NB110-68136] - SK-MEL-28 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Tenascin C at 5 ug/ml overnight at 4C and detected with an anti-Mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) [NB110-68136] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse bone-tendon using Tenascin C antibody (clone 4C8MS) at 1:25 dilution. The signal was detected using HRP-DAB detection method which followed counterstaining using hematoxylin. The antibody generated a very specific cytoplasmic, membrane and extra-cellular signal in tendon fibroblasts, osteoblasts, osteoclasts, and some bone marrow cells. The mineralized areas were largely negative for the staining.
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1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
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Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - A surface stain was performed on HeLa cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
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Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.
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Publications for Fibronectin Antibody (NBP2-32851)(1)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 1 application: IHC.


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Fibronectin Antibody (NBP2-32851) (0)

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Control Lysate(s)

Fibronectin Knockout HeLa Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34526AF350
Alexa Fluor 405 NBP2-34526AF405
Alexa Fluor 488 NBP2-34526AF488
Alexa Fluor 532 NBP2-34526AF532
Alexa Fluor 594 NBP2-34526AF594
Alexa Fluor 647 NBP2-34526AF647
Alexa Fluor 700 NBP2-34526AF700
Alexa Fluor 750 NBP2-34526AF750
Allophycocyanin NBP2-34526APC
Allophycocyanin/Cy7 NBP2-34526APCCY7
Biotin NBP2-34526B
DyLight 350 NBP2-34526UV
DyLight 405 NBP2-34526V
DyLight 488 NBP2-34526G
DyLight 550 NBP2-34526R
DyLight 594 NBP2-34526DL594
DyLight 650 NBP2-34526C
DyLight 680 NBP2-34526FR
DyLight 755 NBP2-34526IR
FITC NBP2-34526F
HRP NBP2-34526H
Janelia Fluor 549 NBP2-34526JF549
Janelia Fluor 646 NBP2-34526JF646
PE NBP2-34526PE
PE/Atto594 NBP2-34526PEATT594
PE/Cy5.5 NBP2-34526PECY55
PE/Cy7 NBP2-34526PECY7
PerCP NBP2-34526PCP
Learn about Custom Antibody Labeling

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Check out the latest blog posts on Fibronectin.
Nickel induces migratory and invasive phenotype in human epithelial cells by epigenetically activating ZEB1
By Jamshed Arslan Pharm.D. Nickel (Ni) is a naturally abundant metallic element. It is a major component of stainless steel, coins, and many other items of daily use. Disturbingly, Ni exposure is associated with can...  Read full blog post.
Bad news for stomach cancer: BAMBI protein inhibits gastric carcinoma via TGF-beta/epithelial-mesenchymal transition signaling
By Jamshed Arslan Pharm.D. Gastric carcinoma is the second leading cause of cancer-related deaths worldwide. One of the key features of gastric carcinoma is acidosis, which promotes growth and metastasis of gastric...  Read full blog post.
Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.
Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.
Fibronectin: Organizing Cell Activity across the ECM
Fibronectin is a glycoprotein found in the extracellular matrix (ECM) that binds to integrins and other components of the ECM such as collagen and fibrin. Under normal physiological conditions, fibronectin is an important factor in cell adhesion, grow...  Read full blog post.
Using the Laminin Antibody in Angiogenesis Research
Laminin is one of a large number of proteins expressed on the basal laminar of the ECM (extracellular matrix). The laminin antibody database covers several proteins, which interact with integrins and other receptor proteins to support cellular differe...  Read full blog post.

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Bioinformatics

Gene Symbol FN1
Uniprot