FHL2 Knockout HeLa Cell Lysate

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Concentration
LYOPH

Order Details

FHL2 Knockout HeLa Cell Lysate Summary

Preparation
Method
Knockout achieved by using CRISPR/Cas9,1 bp insertion in exon3 and -1 bp deletion in exon3
Gene
FHL2

Applications/Dilutions

Application Notes
You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
Concentration
LYOPH
Reconstitution Instructions
To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

Type
Knockout HeLa Cell

Notes

Powered by EDIGENE.
Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for FHL2 Knockout HeLa Cell Lysate

  • AAG11
  • down-regulated in rhabdomyosarcoma LIM protein
  • DRAL
  • DRALAAG11
  • FHL2
  • FHL-2
  • four and a half LIM domains 2
  • four and a half LIM domains protein 2
  • four and a half LIM-domain protein 2
  • LIM domain protein DRAL
  • Skeletal muscle LIM-protein 3
  • SLIM3
  • SLIM-3
  • SLIM3aging-associated gene 11

Background

This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. This protein is thought to have a role in the assembly of extracellular membranes. Also, this gene is down-regulated during transformation of normal myoblasts to rhabdomyosarcoma cells and the encoded protein may function as a link between presenilin-2 and an intracellular signaling pathway. Multiple alternatively spliced variants encoding different isoforms have been identified. [provided by RefSeq, Jan 2016]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol FHL2
Entrez