FHL2 Antibody [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

FHL2 Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human FHL2
  Lys167-Ile279
  Accession # Q14192
• Specificity: Detects human FHL2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human FHL1 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FHL2 Antibody [Alexa Fluor® 647]

• AAG11
• down-regulated in rhabdomyosarcoma LIM protein
• DRAL
• DRALAAG11
• FHL2
• FHL-2
• four and a half LIM domains 2
• four and a half LIM domains protein 2
• four and a half LIM-domain protein 2
• LIM domain protein DRAL
• Skeletal muscle LIM-protein 3
• SLIM3
• SLIM-3
• SLIM3aging-associated gene 11

Background

FHL2 (Four-and-a-half LIM domains protein 2; also SLIM3 and DRAL) is a 30‑32 kDa member of the four-and-a half class of the LIM domain-only protein family. It shows a tissue-specific expression pattern (myocardium; skeletal muscle; prostatic epithelium), and is noted for its strong transactivation of the androgen receptor (AR). Human FHL2 is 279 amino acids (aa) in length. It contains four distinct LIM domains (aa 40‑92; 101‑153; 162‑212; 221‑275) that all bind to the AR. There are two potential alternate start sites (Met115 and Met110) and one splice variant that shows a 99 aa substitution for aa 53‑279. Over aa 167‑279, human FHL2 is 95% and 96% aa identical to canine and mouse FHL2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.