Fgr Overexpression Lysate

Images

 
Western Blot: FGR Overexpression Lysate (Adult Normal) [NBL1-10712] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FGR.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Fgr Overexpression Lysate Summary

Description

Fgr Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC214458

Accession#: NM_005248

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
FGR

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
59.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Fgr Overexpression Lysate

  • c-fgr
  • c-src-2 proto-oncogene
  • c-src2
  • EC 2.7.10
  • EC 2.7.10.2
  • Fgr
  • FLJ43153
  • Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog
  • MGC75096
  • p55-c-fgr protein
  • p55c-fgr
  • p55-Fgr
  • p58c-fgr
  • Proto-oncogene c-Fgr
  • proto-oncogene tyrosine-protein kinase FGR
  • Src2
  • SRC2c-fgr protooncogene
  • tyrosine-protein kinase Fgr

Background

This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to plasma membrane ruffles, and functions as a negative regulator of cell migration and adhesion triggered by the beta-2 integrin signal transduction pathway. Infection with Epstein-Barr virus results in the overexpression of this gene. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]. Transcript Variant: This variant (1) initiates at exon M4 and represents the longest transcript. It is expressed in myelomonocytic cells. Variants 1, 2, and 3 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for Fgr Lysate (NBL1-10712) (0)

There are no publications for Fgr Lysate (NBL1-10712).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Fgr Lysate (NBL1-10712) (0)

There are no reviews for Fgr Lysate (NBL1-10712). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Fgr Lysate (NBL1-10712) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Fgr Products

Research Areas for Fgr Lysate (NBL1-10712)

Find related products by research area.

Blogs on Fgr

There are no specific blogs for Fgr, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Fgr Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol FGR