FGFR3 Antibody (2H10B4) - BSA Free

Western Blot: FGFR3 Antibody (2H10B4) [NBP2-52468] - Analysis using FGFR3 mAb against human FGFR3 (AA: 529-694) recombinant protein. (Expected MW is 44.1 kDa)

Immunohistochemistry: FGFR3 Antibody (2H10B4) [NBP2-52468] - Analysis of ovarian cancer tissues using FGFR3 mouse mAb with DAB staining.

Flow Cytometry: FGFR3 Antibody (2H10B4) [NBP2-52468] - Analysis of A549 cells using FGFR3 mouse mAb (green) and negative control (red).

Western Blot: FGFR3 Antibody (2H10B4) [NBP2-52468] - Analysis using FGFR3 mAb against HEK293 (1) and FGFR3 (AA: 529-694)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry-Paraffin: FGFR3 Antibody (2H10B4) [NBP2-52468] - Analysis of endometrial cancer tissues using FGFR3 mouse mAb with DAB staining.

ELISA: FGFR3 Antibody (2H10B4) [NBP2-52468] - Black line: Control Antigen (100 ng);Purple line: Antigen(10ng);Blue line: Antigen (50 ng);Red line: Antigen (100 ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC, CyTOF-ready
• Clone: 2H10B4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

FGFR3 Antibody (2H10B4) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human FGFR3 (AA: 529-694) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FGFR3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500-1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 87.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for FGFR3 Antibody (2H10B4) - BSA Free

• CD333
• CEK
• EC 2.7.10
• FGF R3
• FGFR3
• fibroblast growth factor receptor 3
• HSFGFR3EX
• JTK4
• JTK4thanatophoric dwarfism

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FGFR3 Antibody (NBP2-52468)(2)

Publications using NBP2-52468

• F De Bacco, F Orzan, J Erriquez, E Casanova, L Barault, R Albano, A D'Ambrosio, V Bigatto, G Reato, M Patanè, B Pollo, G Kuesters, C Dell'Aglio, L Casorzo, S Pellegatta, G Finocchiar, PM Comoglio, C Boccaccio ERBB3 overexpression due to miR-205 inactivation confers sensitivity to FGF, metabolic activation, and liability to ERBB3 targeting in glioblastoma Cell Reports, 2021-07-27;36(4):109455. 2021-07-27 [PMID: 34320350]
• Sohni A, Tan K, Song HW et al. The Neonatal and Adult Human Testis Defined at the Single-Cell Level Cell Rep 2019-02-05 [PMID: 30726734] (IHC-P, Human)

Bioinformatics

• Gene Symbol: FGFR3