FGFBP2 Antibody [Unconjugated]

FGFBP2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Sheep Anti-Human FGFBP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9349) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated

FGFBP2 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human FGFBP2
  Gln20-Gly223
  Accession # Q9BYJ0
• Specificity: Detects human FGFBP2 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 1-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGFBP2 Antibody [Unconjugated]

• 37 kDa killer-specific secretory protein
• FGF-Binding Protein 2
• FGFBP2
• FGF-BP2
• FGFBP-2
• fibroblast growth factor binding protein 2
• fibroblast growth factor-binding protein 2
• HBP17RP
• HBp17-RP
• killer-specific secretory protein of 37 kDa
• KSP37
• KSP37HBp17-related protein

Background

Fibroblast growth factor-binding protein 2 (FGFBP2) also known as killer-specific secretory protein (Ksp37) belongs to the fibroblast growth factor binding protein family. This is a 37kDa Th1-specific serum protein secreted by NK cells. There is a correlation between elevated levels of FGFBP2 and infection development indicating that this protein is involved with cytotoxic lymphocyte-mediated immunity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FGFBP2 Antibody (AF9349)(2)

Publications using AF9349

• Tianyu Fan, Jian Lu, Delei Niu, Yue Zhang, Bin Wang, Bei Zhang et al. Immune and non-immune cell subtypes identify novel targets for prognostic and therapeutic strategy: A study based on intratumoral heterogenicity analysis of multicenter scRNA-seq datasets in lung adenocarcinoma Frontiers in Immunology 11/22/2022 [PMID: 36483553]
• HJ Park, MS Shin, M Kim, JB Bilsborrow, S Mohanty, RR Montgomery, AC Shaw, S You, I Kang Transcriptomic analysis of human IL-7 receptor alpha low and high effector memory CD8+ T cells reveals an age-associated signature linked to influenza vaccine response in older adults Aging Cell, 2019-05-01;0(0):e12960. 2019-05-01 [PMID: 31044512] (Flow Cytometry, Human)

Bioinformatics

• Uniprot:
  • Q9BYJ0()