FGF-BP Antibody

FGF-BP was detected in perfusion fixed frozen sections of rat thymus using Rat FGF-BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1413) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Rt
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

FGF-BP Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat FGF-BP
  Glu21-Cys238
  Accession # Q9QY10
• Specificity: Detects rat FGF-BP in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human FGF-BP is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FGFBP1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGF-BP Antibody

• 17 kDa heparin-binding growth factor-binding protein
• FGFBP
• FGF-BP
• FGFBP1
• FGF-BP1
• FGFBP-1
• FGFBPFGF-binding protein 1
• fibroblast growth factor binding protein 1
• fibroblast growth factor-binding protein 1
• HBp17
• HBP1717 kDa HBGF-binding protein
• heparin-binding growth factor binding protein

Background

Fibroblast growth factor binding protein (FGF-BP), also known as HBp17, is a secreted glycoprotein that increases the bioavailability of FGFs (1). Mature FGF-BP is a 34 kDa O-glycosylated protein with five conserved intrachain disulfide bonds (2-4). FGF-BP contains a heparin-binding domain (aa 113-146) and a distinct FGF-binding region (aa 197-238) (5). Mature rat FGF-BP shares 54% and 81% aa sequence identity with mouse and rat FGF-BP, respectively. FGF-BP is expressed throughout development and in adult squamous epithelium (6, 7). It is upregulated in injured skin, renal tubular epithelium, and spinal nerves as well as in carcinomas of the skin, colon, and pancreas (3, 8-11). FGF-BP binds FGF -1, -2, -7, -10, and -22 which are secreted and sequestered in the extracellular matrix (ECM) (8, 12). The interactions of FGF-BP with heparin sulfate proteoglycans (HSPG) and FGF, modulates their activities (7, 9 13, 14). FGF-BP enhances the mitogenic effects of FGFs, thereby contributing to epithelial, endothelial, and neuronal tissue repair, angiogenesis, and tumor growth (8-10, 12, 15, 16).

 

1. Abuharbeid, S. et al. (2006) Int. J. Biochem. Cell Biol. 38:1463.
2. Aigner, A. et al. (2000) Growth Factors 18:51.
3. Tassi, E. et al. (2006) Cancer Res. 66:1191.
4. Lametsch, R. et al. (2000) J. Biol. Chem. 275:19469.
5. Xie, B. et al. (2006) J. Biol. Chem. 281:1137.
6. Aigner, A. et al. (2002) Histochem. Cell Biol. 117:1.
7. Wu, D. et al. (1991) J. Biol. Chem. 266:16778.
8. Beer, H-D. et al. (2005) Oncogene 24:5269.
9. Ray, P.E. et al. (2006) Am. J. Physiol. Regul. Integr. Comp. Physiol. 290:R105.
10. Tassi, E. et al. (2007) Am. J. Physiol. Regul. Integr. Comp. Physiol. 293:R775.
11. Kurtz, A. et al. (2004) Neoplasia 6:595.
12. Tassi, E. et al. (2001) J. Biol. Chem. 276:40247.
13. Mongiat, M. et al. (2001) J. Biol. Chem. 276:10263.
14. Kurtz, A. et al. (1997) Oncogene 14:2671.
15. Aigner, A. et al. (2001) Int. J. Cancer 92:510.
16. Czubayko, F. et al. (1997) Nat. Med. 3:1137.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FGF-BP Antibody (AF1413)(1)

Publication using AF1413

• K Nagashima, T Miwa, H Soumiya, D Ushiro, T Takeda-Kaw, N Tamaoki, S Ishiguro, Y Sato, K Miyamoto, T Ohno, M Osawa, T Kunisada, T Shibata, KI Tezuka, S Furukawa, H Fukumitsu Priming with FGF2 stimulates human dental pulp cells to promote axonal regeneration and locomotor function recovery after spinal cord injury Sci Rep, 2017-10-18;7(1):13500. 2017-10-18 [PMID: 29044129] (IHC-Fr, Rat)

Bioinformatics

• Gene Symbol: FGFBP1
• Uniprot:
  • Q9QY10()