FGF-15 Antibody [Alexa Fluor® 350]

• Reactivity: Mu
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 350

FGF-15 Antibody [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant mouse FGF-15
  Ser24-Lys218
  Accession # O35622
• Specificity: Detects mouse FGF-15 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) FGF-19 is observed and less than 5% cross-reactivity with rhFGF-22 and rhFGF-23 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGF-15 Antibody [Alexa Fluor® 350]

• FGF15
• FGF-15
• FGF-19

Background

FGF-15 (Fibroblast growth factor 15) is a 25 kDa, secreted, nonglycosylated polypeptide that is a member of the FGF-15/21/23 subfamily, FGF family of growth factors. It is synthesized by ileal epithelium, colonic myofibroblasts, and select areas of the developing brain. FGF-15 acts on hepatocytes and reduces CYP7A1 levels, thus blocking bile acid production. It also promotes colonic epithelial migration, and in the embryonic brain, inhibits over-production of neuronal precursors. FGF-15 is synthesized as a 218 amino acid (aa) precursor that contains a 25 aa signal sequence and a 182 aa mature region (aa 26-218; 23 kDa predicted MW). Mature FGF-15 contains a characteristic 3-dimensional beta -trefoil structure, plus a unique Cys pattern that contributes to stability. There are two potential splice forms. One shows a 23 aa substitution for aa 120-218, while a second shows a 41 aa substitution for aa 120-218. Over aa 23-200, mouse FGF-15 shares 96% aa identity with rat FGF-15. Although mouse FGF-15 is considered the ortholog to human FGF-19, the two molecules share only 54% aa identity over aa 23-200, and are produced by different cell types (intestinal epithelium in mouse, hepatocytes in human).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.