FGF-12 Antibody (1D9) - Azide and BSA Free

Western Blot: FGF-12 Antibody (1D9) [H00002257-M10] - Analysis of FGF12 expression in transfected 293T cell line by FGF12 monoclonal antibody (M10), clone 1D9. Lane 1: FGF12 transfected lysatE (27.4 KDa). Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: FGF-12 Antibody (1D9) [H00002257-M10] - Analysis of monoclonal antibody to FGF12 on formalin-fixed paraffin-embedded human cerebral cortex. Antibody concentration 3 ug/ml

ELISA: FGF-12 Antibody (1D9) [H00002257-M10] - Detection limit for recombinant GST tagged FGF12 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 1D9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

FGF-12 Antibody (1D9) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: FGF12 (AAH22524, 1 a.a. ~ 181 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MESKEPQLKGIVTRLFSQQGYFLQMHPDGTIDGTKDENSDYTLFNLIPVGLRVVAIQGVKASLYVAMNGEGYLYSSDVFTPECKFKESVFENYYVIYSSTLYRQQESGRAWFLGLNKEGQIMKGNRVKKTKPSSHFVPKPIEVCMYREQSLHEIGEKQGRSRKSSGTPTMNGGKVVNQDST
• Specificity: FGF12 (1D9)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FGF12
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for FGF-12 Antibody (1D9) - Azide and BSA Free

• FGF12
• FGF-12
• FGF12B
• FHF1
• FHF-1
• FHF1Fibroblast growth factor homologous factor 1
• fibroblast growth factor 12
• fibroblast growth factor 12B
• fibroblast growth factor FGF-12b
• Myocyte-activating factor

Background

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This growth factor lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfected into mammalian cells, this protein accumulated in the nucleus, but was not secreted. The specific function of this gene has not yet been determined. Two alternatively spliced transcript variants encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FGF-12 Antibody (H00002257-M10)(2)

Publications using H00002257-M10

• Bhushan A, Singh A, Kapur S et al. Identification and Validation of Fibroblast Growth Factor 12 Gene as a Novel Potential Biomarker in Esophageal Cancer Using Cancer Genomic Datasets. OMICS 2017-10-01 [PMID: 29049013]
• Chattopadhyay I, Singh A, Phukan R et al. Genome-wide analysis of chromosomal alterations in patients with esophageal squamous cell carcinoma exposed to tobacco and betel quid from high-risk area in India. Mutat Res;696(2):130-138. 2010-02-02 [PMID: 20083228]

Bioinformatics

• Gene Symbol: FGF12
• Entrez:
  • 2257(Human)
• OMIM:
  • 601513(Human)
• Uniprot:
  • AAH22524(Human)