JIP2 Antibody

Images

 
Western Blot: JIP2 Antibody [NBP1-31411] - Sample(30 ug whole cell lysate) A:Hep G2 7.5% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: JIP2 Antibody [NBP1-31411] - Analysis of A431, using NBP1-31411 at 1:200 dilution.
Immunohistochemistry-Paraffin: JIP2 Antibody [NBP1-31411] - Paraffin-embedded lung SCC xenograft, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

JIP2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human JIP1. The exact sequence is proprietary.
Localization
Cytoplasm, Cytoplasm, perinuclear region, Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAPK8IP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for JIP2 Antibody

  • C-Jun-amino-terminal kinase-interacting protein 2
  • homologous to mouse JIP-1
  • IB-2
  • IB2JIP2Mitogen-activated protein kinase 8-interacting protein 2
  • islet-brain 2
  • islet-brain-2
  • JIP-2
  • JNK MAP kinase scaffold protein 2
  • JNK MAP kinase scaffold protein JIP2
  • JNK-interacting protein 2
  • mitogen-activated protein kinase 8 interacting protein 2
  • PRKM8 interacting protein-like
  • PRKM8IPL

Background

c-Jun NH2-terminal kinases (JNKs) are distant members of the MAP kinase family. JNK1 is activated by dual phosphorylation at a Thr-Pro-Tyr motif in response to ultraviolet (UV) light, and it functions to phosphorylate c-Jun at amino terminal serine regulatory sites, Ser 63 and Ser 73, resulting in transcriptional activation. Two additional JNK family members have been identified as JNK2 and JNK3. JIP-1 (for JNK interacting protein-1) has been identified as a cytoplasmic inhibitor of JNK that retains JNK in the cytoplasm, thereby inhibiting JNK-regulated gene expression. Evidence suggests that JNK1 and JNK2 bind to JIP-1 with greater affinity than to ATF-2 and c-Jun, which are targets of the JNK signaling pathway. JIP-1 contains an amino terminal JNK binding domain and a carboxy terminal SH3 domain. ATF-2 and c-Jun also contain the JNK binding domain and are thought to compete with JIP-1 for JNK binding. Multiple splice variants of JIP-1, including JIP-1b, JIP-1c (also designated islet-brain 1 or IB-1), JIP-2a, JIP-2b and JIP-3, have been identified in brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional JIP2 Products

Array NBP1-31411

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Bioinformatics

Gene Symbol MAPK8IP2