JIP2 Antibody - BSA Free

Western Blot: JIP2 Antibody [NBP1-31411] - Sample(30 ug whole cell lysate) A:Hep G2 7.5% SDS PAGE, antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: JIP2 Antibody [NBP1-31411] - Paraffin-embedded lung SCC xenograft, using antibody at 1:100 dilution.

Immunohistochemistry: JIP2 Antibody [NBP1-31411] - JIP2 antibody detects JIP2 protein at cytoplasm on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. JIP2 antibody (NBP1-31411) diluted at 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

JIP2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit JIP2 Antibody - BSA Free (NBP1-31411) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human JIP2. The exact sequence is proprietary.
• Localization: Cytoplasm, Cytoplasm, perinuclear region, Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAPK8IP2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for JIP2 Antibody - BSA Free

• C-Jun-amino-terminal kinase-interacting protein 2
• homologous to mouse JIP-1
• IB-2
• IB2JIP2Mitogen-activated protein kinase 8-interacting protein 2
• islet-brain 2
• islet-brain-2
• JIP-2
• JNK MAP kinase scaffold protein 2
• JNK MAP kinase scaffold protein JIP2
• JNK-interacting protein 2
• mitogen-activated protein kinase 8 interacting protein 2
• PRKM8 interacting protein-like
• PRKM8IPL

Background

c-Jun NH2-terminal kinases (JNKs) are distant members of the MAP kinase family. JNK1 is activated by dual phosphorylation at a Thr-Pro-Tyr motif in response to ultraviolet (UV) light, and it functions to phosphorylate c-Jun at amino terminal serine regulatory sites, Ser 63 and Ser 73, resulting in transcriptional activation. Two additional JNK family members have been identified as JNK2 and JNK3. JIP-1 (for JNK interacting protein-1) has been identified as a cytoplasmic inhibitor of JNK that retains JNK in the cytoplasm, thereby inhibiting JNK-regulated gene expression. Evidence suggests that JNK1 and JNK2 bind to JIP-1 with greater affinity than to ATF-2 and c-Jun, which are targets of the JNK signaling pathway. JIP-1 contains an amino terminal JNK binding domain and a carboxy terminal SH3 domain. ATF-2 and c-Jun also contain the JNK binding domain and are thought to compete with JIP-1 for JNK binding. Multiple splice variants of JIP-1, including JIP-1b, JIP-1c (also designated islet-brain 1 or IB-1), JIP-2a, JIP-2b and JIP-3, have been identified in brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: MAPK8IP2