FFAR2/GPR43 Antibody (1000624) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with (A) human FFAR2/GPR43 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human FFAR2/GPR43 Monoclonal Antibody (Catalog # MAB10082) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody(Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 1000624
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

FFAR2/GPR43 Antibody (1000624) [Unconjugated] Summary

• Immunogen: NS0 Mouse myeloma cell line transfected with human FFAR2/GPR43
  Met1-Glu330
  Accession # O15552
• Specificity: Stains human FFAR2/GPR43 transfectants but not irrelevant transfectants in flow cytometry.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FFAR2/GPR43 Antibody (1000624) [Unconjugated]

• FFAR2
• free fatty acid activated receptor 2
• free fatty acid receptor 2
• G protein-coupled receptor 43
• GPR43
• GPR43FFA2R
• G-protein coupled receptor 43

Background

Human Free fatty acid receptor 2 (FFA2, FFAR2, GPCR43 or GPR43) is a 330 aminoacids G-protein coupled receptor encoded by the FFAR2 gene. G-protein-coupled receptors (GPCRs) are seven-trans-membrane (7TM) receptors that mediate cellular responses to the majority of hormones and neurotransmitters and are common targets for drug discovery. Human GPR43/FFA2 is present in a large variety of tissues, including adipose tissue, inflammatory cells, and gastrointestinal (GI) tract and is activated by short-chain fatty acids (SCFAs). Studies on knockout mice have identified Free Fatty Acid Receptor 2 (FFAR2 or GPR43) as a critical gene in the prevention of obesity, colitis, asthma and arthritis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O15552(Human)