FEZ1 Antibody - BSA Free

Western Blot: FEZ1 Antibody [NBP1-76395] - Analysis of SAPAP2 in Raji cell lysate with SAPAP2 antibody at (A) 0.5 and (B) 1 ug/mL.

Western Blot: FEZ1 Antibody [NBP1-76395] - SK-N-SH cell lysate with FEZ1 antibody at 1 ug/mL in the (A) presence and (B) absence of blocking peptide.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

FEZ1 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human FEZ1. The immunogen is located within amino acids 270 - 320 of FEZ1. Amino Acid Squence: KQKEQRELMKKRRKEKGL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FEZ1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 0.5-1 ug/ml
• Application Notes: Despite its predicted molecular weight, FEZ1 will often run at higher molecular weight in SDS-PAGE.
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  FEZ1 Peptide (NBP1-76395PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for FEZ1 Antibody - BSA Free

• fasciculation and elongation protein zeta 1 (zygin I)
• fasciculation and elongation protein zeta-1
• Zygin I
• zygin-1

Background

Similar to its C. elegans homolog UNC-76, mammalian FEZ1, an abundant membrane protein and protein kinase C (PKC)-Z substrate, is involved in axonal guidance. Following phosphorylation by PKC-Z, FEZ1 translocates from the plasma membrane to the cytoplasm. FEZ1 is then able to interact with a number of proteins involved in axonal outgrowth and cellular transport such as DISC1, a candidate gene for schizophrenia, as well as kinesin-1, a microtubule-based motor protein. FEZ1 also interacts with proteins involved in transcriptional regulation and chromatin remodeling, suggesting that FEZ1 may have nuclear regulatory functions in addition to those for neuronal development. At least two distinct isoforms of FEZ1 are known to exist. Despite its predicted molecular weight, FEZ1 will often run at higher molecular weight in SDS-PAGE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FEZ1
• Uniprot:
  • Q99689(Human)