Ferroportin/SLC40A1 Antibody - Azide and BSA Free

Western Blot: Ferroportin/SLC40A1 Antibody [H00030061-D01P] - Analysis of Ferroportin/SLC40A1 expression (Molecular weight: 62.5 KDa) in transfected 293T cell line by polyclonal Ferroportin/SLC40A1 Antibody.Lane 1: Ferroportin/SLC40A1 transfected lysate(62.50 KDa).Lane 2: Non-transfected lysate.

Western Blot: Ferroportin/SLC40A1 Antibody [H00030061-D01P] - Analysis of Ferroportin/SLC40A1 (Molecular weight: 62.5 KDa) expression in A-431 using Ferroportin/SLC40A1 Antibody.

Western Blot: Ferroportin/SLC40A1 Antibody [H00030061-D01P] - Analysis of Ferroportin/SLC40A1 expression (Molecular weight: 62.5 KDa) in human liver using Ferroportin/SLC40A1 Antibody.

Western Blot: Ferroportin/SLC40A1 Antibody [H00030061-D01P] - Analysis of Ferroportin/SLC40A1 expression (Molecular weight: 62.5 KDa) in IMR-32 using Ferroportin/SLC40A1 Antibody.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Ferroportin/SLC40A1 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Ferroportin/SLC40A1 Antibody - Azide and BSA Free (H00030061-D01P) is a polyclonal antibody validated for use in WB. Anti-Ferroportin/SLC40A1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: SLC40A1 (NP_055400.1, 1 a.a. - 571 a.a.) full-length human protein. MTRAGDHNRQRGCCGSLADYLTSAKFLLYLGHSLSTWGDRMWHFAVSVFLVELYGNSLLLTAVYGLVVAGSVLVLGAIIGDWVDKNARLKVAQTSLVVQNVSVILCGIILMMVFLHKHELLTMYHGWVLTSCYILIITIANIANLASTATAITIQRDWIVVVAGEDRSKLANMNATIRRIDQLTNILAPMAVGQIMTFGSPVIGCGFISGWNLVSMCVEYVLLWKVYQKTPALAVKAGLKEEETELKQLNLHKDTEPKPLEGTHLMGVKDSNIHELEHEQEPTCASQMAEPFRTFRDGWVSYYNQPVFLAGMGLAFLYMTVLGFDCITTGYAYTQGLSGSILSILMGASAITGIMGTVAFTWLRRKCGLVRTGLISGLAQLSCLILCVISVFMPGSPLDLSVSPFEDIRSRFIQGESITPTKIPEITTEIYMSNGSNSANIVPETSPESVPIISVSLLFAGVIAARIGLWSFDLTVTQLLQENVIESERGIINGVQNSMNYLLDLLHFIMVILAPNPEAFGLLVLISVSFVAMGHIMYFRFAQNTLGNKLFACGPDAKEVRKENQANTSVV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC40A1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Antibody reactive against mammalian transfected lysate.
• Theoretical MW: 62.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein G purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Ferroportin/SLC40A1 Antibody - Azide and BSA Free

• Ferroportin
• Ferroportin-1
• FPN1
• FPN1IREG1ferroportin 1
• HFE4
• HFE4ferroportin-1
• IREG1
• iron regulated gene 1
• Iron-regulated transporter 1
• member 3
• MST079
• MSTP079
• MTP1
• putative ferroportin 1 variant IIIB
• SLC11A3
• SLC11A3iron regulated gene 1
• SLC40A1
• solute carrier family 11 (proton-coupled divalent metal ion transporters)
• solute carrier family 11 (proton-coupled divalent metal ion transporters), member 3
• solute carrier family 40 (iron-regulated transporter), member 1
• solute carrier family 40 member 1

Background

Ferroportin is a 12-transmembrane domain protein, belonging to the major facilitator superfamily of transporters of small molecules, that is localized to the plasma membrane. Human Ferroportin has a theoretical molecular weight of 62.5 kDa. Ferroportin (FPN1 or SLC40A1) functions as an iron-regulated transporter (highly expressed in placenta, intestine, muscle, spleen, macrophages etc.) and is the receptor for the iron-regulatory hormone, hepcidin. In iron metabolism, FPN1 plays a key role in intestinal iron absorption as well as cellular iron release and mediates iron absorption in the presence of ferroxidases, hephaestin (HP) and/or ceruloplasmin (CP). FPN1 is implicated in iron export from duodenal epithelial cells and in the transfer of iron between maternal and fetal circulation. FPN1 transports iron in the ferrous form whereas plasma transferrin only binds iron's ferric form. Ferroxidases are key players in oxidizing iron transported by FPN1 and without the activity of ferroxidases, FPN1 is internalized followed by degradation. While other cell types utilize the circulating or GPI-linked multicopper ferroxidase CP for FPN1, intestinal cells utilize a membrane-bound HP, a paralog of CP that also show interaction with FPN1 (1).

FPN1 regulation is dependent on the cell type and involves transcriptional, posttranscriptional, and posttranslational mechanisms including hepcidin-mediated endocytosis and proteolysis. Hepcidin controls the concentration of FPN1 in the membrane, with hepcidin deficiency resulting in iron overload (high iron) and hepcidin excess leading to iron restriction and anemia (2). Ferroportin disease or hemochromatosis type 4 (HFE4) is associated with distinct FPN1 variants with either reduced FPN1 cell surface expression/iron export capacity or hepcidin resistance and iron overload (3, 4).

References

1. De Domenico I, Ward DM, Kaplan J. (2011) Hepcidin and ferroportin: the new players in iron metabolism. Semin Liver Dis. 31(3):272-9. PMID: 21901657

2. Drakesmith H, Nemeth E, Ganz T. (2015) Ironing out Ferroportin. Cell Metab. 22(5):777-87. PMID: 26437604

3. Pietrangelo A. (2017) Ferroportin disease: pathogenesis, diagnosis and treatment. Haematologica. 102(12):1972-1984. PMID: 29101207

4. Vlasveld LT, Janssen R, Bardou-Jacquet E, Venselaar H, Hamdi-Roze H, Drakesmith H, Swinkels DW. (2019) Twenty Years of Ferroportin Disease: A Review or An Update of Published Clinical, Biochemical, Molecular, and Functional Features. Pharmaceuticals (Basel). 12(3). pii: E132. PMID: 31505869

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ferroportin/SLC40A1 Antibody (H00030061-D01P)(1)

Publication using H00030061-D01P

• Michela A, Luca C, Magdalena G et al. The modulation of iron metabolism affects the Rhabdomyosarcoma tumor growth in vitro and in vivo. Clin Exp Med. 2023-02-10 [PMID: 36764998]

Bioinformatics

• Gene Symbol: SLC40A1
• Uniprot:
  • NP_055400.1(Human)