Ferritin Heavy Chain Antibody (HL1905)

Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [HL1905] (NBP3-48666) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [HL1905] (NBP3-48666) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 15% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [HL1905] (NBP3-48666) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [HL1905] (NBP3-48666) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Ca, Fe
• Applications: WB
• Clone: HL1905
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Ferritin Heavy Chain Antibody (HL1905) Summary

• Description: Novus Biologicals Rabbit Ferritin Heavy Chain Antibody (HL1905) (NBP3-48666) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human Ferritin Heavy Chain.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FTH1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Ferritin Heavy Chain Antibody (HL1905)

• apoferritin
• Cell proliferation-inducing gene 15 protein
• Ferritin H subunit
• ferritin heavy chain
• ferritin, heavy polypeptide 1
• FHC
• FTHEC 1.16.3.1
• FTHL6MGC104426
• PIG15
• placenta immunoregulatory factor
• PLIF
• proliferation-inducing protein 15

Background

Stores iron in a soluble. nontoxic. readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Ferritin is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis by sequestering and storing iron in a non-toxic and soluble form. It forms a holoenzyme of ~450 kDa, consisting of 24 subunits of two types, H (heavy; 21 kDa) and L (light; 19 kDa), and is capable of storing up to 4,500 atoms of ferric iron. Depending on the tissue type and physiological status of the cell, the ratio of H to L subunits in ferritin can vary widely. Ferritin is found in the liver, spleen, kidney and heart, with smaller amounts being found in blood. Serum ferritin levels serve as an indicator of the amount of iron stored in the body. Serum ferritin is the most sensitive test for anaemia, and is also used as a marker for restless leg syndrome, hemochromatosis and porphyria. As ferritin is an acute-phase reactant, it is often elevated during infection. Defects in ferritin proteins are associated with several neurodegenerative diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FTH1