Ferritin Antibody - BSA Free

Western Blot: Ferritin Antibody [NB600-920] - Lane 1: Ferritin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Ferritin primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 20 kDa, 20 kDa for Ferritin. Other band(s): None.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: LYOPH

Ferritin Antibody - BSA Free Summary

• Description: This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ferritin [Human Spleen]
  
  Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: Ferritin [Human Spleen] (Uniprot: P02794)
• Specificity: Cross reactivity against Ferritin Heavy Chain from other tissues and species may occur but have not been specifically determined.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FTH1
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:20000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:5000-1:2000
• Application Notes: This product has been tested in ELISA and western blot and is suitable for use in immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
• Control:

  Ferritin Light Chain Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent)

Alternate Names for Ferritin Antibody - BSA Free

• apoferritin
• Cell proliferation-inducing gene 15 protein
• Ferritin H subunit
• ferritin heavy chain
• Ferritin
• ferritin, heavy polypeptide 1
• FHC
• FTHEC 1.16.3.1
• FTHL6MGC104426
• PIG15
• placenta immunoregulatory factor
• PLIF
• proliferation-inducing protein 15

Background

This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ferritin Antibody (NB600-920)(9)

Publications using NB600-920

• Sen S, Bal SK, Yadav S et al. Intracellular pathogen Leishmania intervenes in iron loading into ferritin by cleaving chaperones in host macrophages as an iron acquisition strategy The Journal of biological chemistry 2022-10-26 [PMID: 36309090] (IP, WB, Mouse)
• Katsarou A, Gkouvatsos K, Fillebeen C, Pantopoulos K Tissue-Specific Regulation of Ferroportin in Wild-Type and Hjv-/- Mice Following Dietary Iron Manipulations Hepatol Commun 2021-09-24 [PMID: 34558857]
• Katsarou A, Gkouvatsos K, Fillebeen C, Pantopoulos K Tissue-Specific Regulation of Ferroportin in Wild-Type and Hjv/Mice Following Dietary Iron Manipulations Hepatology Communications 2021-07-16 [PMID: 34558857] (WB)
• Nobuta H, Yang N, Ng YH et al. Oligodendrocyte Death in Pelizaeus-Merzbacher Disease Is Rescued by Iron Chelation. Cell Stem Cell 2019-10-03 [PMID: 31585094] (WB, Human)
• Kent P, Wilkinson N, Constante M et al. Hfe and Hjv exhibit overlapping functions for iron signaling to hepcidin. J Mol Med (Berl). 2015-01-01 [PMID: 25609138] (WB, Mouse)
• Song W, Zukor H, Lin SH et al. Unregulated brain iron deposition in transgenic mice over-expressing HMOX1 in the astrocytic compartment J Neurochem 2012-11-01 [PMID: 22881289] (WB, Mouse)
• Daba A, Koromilas AE, Pantopoulos K. Alternative ferritin mRNA translation via internal initiation. RNA;18(3):547-56. 2012-03-01 [PMID: 22271759]
• Hausmann A, Lee J, Pantopoulos K. Redox control of iron regulatory protein 2 stability. FEBS Lett;585(4):687-692. 2011-02-18 [PMID: 21281640]
• Hasan MR, Tosha T, Theil EC. Ferritin Contains Less Iron (59Fe) in Cells When the Protein Pores Are Unfolded by Mutation. J Biol Chem;283(46):31394-31400. 2008-01-01 [PMID: 18805796]

Bioinformatics

• Gene Symbol: FTH1
• Entrez:
  • 2495(Human)
• Uniprot:
  • P02794()
  • P02794()
  • P02794()
  • P02794()
  • P02794()