Western Blot: Ferritin Antibody [NB600-920] - Lane 1: Ferritin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Ferritin primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary ...read more
Immunoprecipitation: Ferritin Antibody [NB600-920] - Proteins extracts (1000ug) of HT1080 fibrosarcoma cells were subjected to immunoprecipitation using 5ul of NB600-920 anti-ferritin antibody.
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Ferritin [Human Spleen]
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ferritin [Human Spleen]. Cross-reactivity against Ferritin from other tissues and species may occur but have not been specifically determined.
Ion exchange chromatography
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Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% Sodium Azide
Ion exchange chromatography
Reconstitute to a final concentration of 1mg/ml with deionized water (or equivalent)
Alternate Names for Ferritin Antibody
Cell proliferation-inducing gene 15 protein
Ferritin H subunit
ferritin heavy chain
ferritin, heavy polypeptide 1
placenta immunoregulatory factor
proliferation-inducing protein 15
Ferritin is the major intracellular iron storage protein in all organisms. It has the shape of a hollow sphere that permits entry of a variable amount of iron for storage as ferric hydroxide phosphate complexes. Mammalian liver and spleen ferritin (relative mass about 450,000) consists of 24 subunits of 2 species, the heavy subunit (relative mass = 21,000) and the light subunit (relative mass = 19,000). In models of spinal cord injury ferritin is found in activated microglial cells.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Transferrin and the blood brain barrier Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i... Read full blog post.
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