FCRN/FCGRT Antibody (937508) [Unconjugated]

Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human FCRN Monoclonal Antibody (Catalog # MAB8639) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FCRN at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human FCRN Monoclonal Antibody (Catalog # MAB8639, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: WB, CyTOF-ready, Flow
• Clone: 937508
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

FCRN/FCGRT Antibody (937508) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human FCRN
  Ala24-Ser297
  Accession # P55899
• Specificity: Detects human FCRN in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FCRN/FCGRT Antibody (937508) [Unconjugated]

• alpha-chain
• Fc fragment of IgG, receptor, transporter, alpha
• FCGRT
• FcRn alpha chain
• FCRN
• FCRNimmunoglobulin receptor, intestinal, heavy chain
• IgG Fc fragment receptor transporter alpha chain
• IgG receptor FcRn large subunit p51
• major histocompatibility complex class I-like Fc receptor
• Neonatal Fc receptor
• neonatal Fc-receptor for Ig

Background

The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells (1, 2). Mature human FCRN consists of a 274 amino acid (aa) extracellular domain (ECD) with two N-terminal alpha domains, one alpha 3/immunoglobulin-like domain, a 23 aa transmembrane segment, and a 44 aa cytoplasmic domain (3). Within the ECD, human FCRN shares 68% aa sequence identity with mouse and rat FCRN. Mouse FCRN binds with high affinity to IgG from mouse, human, rat, rabbit, guinea pig, bovine, and sheep, while human FCRN binds IgG with significantly lower affinity and is much more restricted in terms of species recognition (4). It does not bind the structurally related chicken IgY (5). FCRN additionally binds to albumin, and both it and IgG are bound at pH 5.0 but not at pH 8.0 (3, 6). FCRN associates noncovalently with beta 2-Microglobulin, and this interaction is important for the intracellular trafficking of FCRN (7-10). FCRN cycles between the plasma membrane and acidified intracellular compartments of endothelial cells and epithelial cells (5, 8). It binds endocytosed IgG and albumin in the low pH vesicles and transports them to the plasma membrane for extracellular release at higher pH. This protects IgG and albumin from lysosomal degradation and helps maintain the circulating levels of both proteins (5, 6). This mechanism is involved in the bidirectional transport of IgG across epithelial and endothelial barriers including neonatal IgG absorption in the intestine and fetal uptake of maternal antibodies through the placenta (5, 8, 11, 12). In the kidney, FCRN recycles albumin to the serum but removes IgG from the glomular basement membrane and promotes its excretion into the urine (13, 14). FCRN is also expressed in neutrophils and myeloid antigen presenting cells (7, 15, 16). It can enhance IgG-mediated phagocytosis and antigen presentation by these cells, but it promotes the degradation of opsonizing IgG rather than returning it to the circulation (15, 16).

1. Baker, K. et al. (2009) Semin. Immunopathol. 31:223.
2. Baker, K. et al. (2014) Front. Immunol. 5:408.
3. Story, C.M. et al. (1994) J. Exp. Med. 180:2377.
4. Ober, R.J. et al. (2001) Int. Immunol. 13:1551.
5. Dickinson, B.L. et al. (1999) J. Clin. Invest. 104:903.
6. Chaudhury, C. et al. (2003) J. Exp. Med. 197:315.
7. Simister, N.E. and K.E. Mostov (1989) Nature 337:184.
8. Kobayashi, N. et al. (2002) Am. J. Physiol. Renal Physiol. 282:F358.
9. Praetor, A. and W. Hunziker (2002) J. Cell Sci. 115:2389.
10. Zhu, X. et al. (2001) J. Immunol. 166:3266.
11. Spiekermann, G.M. et al. (2002) J. Exp. Med. 196:303.
12. Firan, M. et al. (2001) Int. Immunol. 13:993.
13. Sarav, M. et al. (2009) J. Am. Soc. Nephrol. 20:1941.
14. Akilesh, S. et al. (2008) Proc. Natl. Acad. Sci. 105:967.
15. Vidarsson, G. et al. (2006) Blood 108:3573.
16. Qiao, S.-W. et al. (2008) Proc. Natl. Acad. Sci. 105:9337.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P55899()