Human Fc gamma RII/CD32 ELISA Kit (Colorimetric)

ELISA: Human Fc gamma RII/CD32 ELISA Kit (Colorimetric) [NBP3-00382] - Samples were spiked with high concentrations of Human Fc gamma RII/CD32 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Fc gamma RII/CD32 ELISA Kit (Colorimetric) [NBP3-00382] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 46.88 - 3000 pg/mL
• Sensitivity: 28.13 pg/mL

Human Fc gamma RII/CD32 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human Fc gamma RII/CD32 in samples. No significant cross-reactivity or interference between Human Fc gamma RII/CD32 and analogues was observed.
• Standard Curve Range: 46.88 - 3000 pg/mL
• Sensitivity: 28.13 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: 5.01%
• Intra-Assay: 5.13%
• Spike Recovery: 89 - 107%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: FCGR2B

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Fc gamma RII/CD32 ELISA Kit (Colorimetric)

• CD32 antigen
• CD32B
• CD32Fc fragment of IgG, low affinity II, receptor for (CD32)
• CDw32
• Fc fragment of IgG, low affinity IIb, receptor (CD32)
• Fc fragment of IgG, low affinity IIb, receptor for (CD32)
• Fc gamma RII
• Fc gamma RIIb
• FCG2
• Fc-gamma RII-b
• FcgammaRIIb
• fc-gamma-RIIb
• FCGR2
• FcgRII
• FcgRIIB
• fcRII-b
• FcRII-c
• IGFR2
• IgG Fc receptor II-b
• low affinity immunoglobulin gamma Fc region receptor II-b

Background

CD32 is a receptor for the Fc region of complexed or aggregated immunoglobulins gamma. This is a low affinity receptor. It is Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in downmodulation of previous state of cell activation triggered via antigen receptors on B cells (BCR), T cells (TCR) or via another Fc receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Fc gamma RII/CD32 ELISA Kit (NBP3-00382). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: FCGR2B