Fc gamma RII/CD32 Antibody (FUN-2)

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Flow Cytometry: Fc gamma RII/CD32 Antibody (FUN-2) [NB100-77781] - Analysis using the FITC conjugate of NB100-77781. Staining of Human peripheral blood lymphocytes.
Flow Cytometry: Fc gamma RII/CD32 Antibody (FUN-2) [NB100-77781] - Human peripheral blood lymphocytes stained with purified FUN-2, followed by anti-mouse IgGs FITC

Product Details

Summary
Product Discontinued
View other related Fc gamma RII/CD32 Primary Antibodies

Order Details


    • Catalog Number
      NB100-77781
    • Availability
      Product Discontinued

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Fc gamma RII/CD32 Antibody (FUN-2) Summary

Immunogen
The immunogen was the full length version of the protein: CD32 (FUN-2)
Specificity
CD32 (FUN-2)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FCGR2B
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry <= 0.5 ug/10^6 cells
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume or 100 microliter of whole blood. Immunohistochemistry-frozen sections. It is recommended that the reagent be titrated for optimal performance for each application.
Positive Control
Fc gamma RIIB/CD32b Lysate (NBL1-10656)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for Fc gamma RII/CD32 Antibody (FUN-2)

  • CD32 antigen
  • CD32B
  • CD32Fc fragment of IgG, low affinity II, receptor for (CD32)
  • CDw32
  • Fc fragment of IgG, low affinity IIb, receptor (CD32)
  • Fc fragment of IgG, low affinity IIb, receptor for (CD32)
  • Fc gamma RII
  • Fc gamma RIIb
  • FCG2
  • Fc-gamma RII-b
  • fc-gamma-RIIb
  • FCGR2
  • FcgRII
  • fcRII-b
  • IGFR2
  • IgG Fc receptor II-b
  • low affinity immunoglobulin gamma Fc region receptor II-b

Background

CD32 is a 40 kD polymorphic transmembrane glycoprotein also known as Fc gammaRII and FCRII. It is an immunoglobulin superfamily member expressed on monocytes/macrophages, granulocytes, platelets and B cells. There are at least 6 isoforms of CD32 resulting from alternative mRNA splicing. CD32 mediates phagocytosis and oxidative burst in granulocytes, as well as platelet aggregation and immunomodulation. The extracellular domain of CD32 binds to polymeric and aggregated IgG and immune complexes, while the intracellular domain has been reported to associate with SHP-1 (B1 isoform).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FCGR2B
Entrez