Fc gamma RII/CD32 Antibody (FCGR2A/479) [Allophycocyanin]

• Reactivity: Hu
• Applications: Flow, ICC/IF
• Clone: FCGR2A/479
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

Fc gamma RII/CD32 Antibody (FCGR2A/479) [Allophycocyanin] Summary

• Immunogen: Recombinant human Fc gamma RII/CD32 protein (Uniprot: P12318)
• Localization: Cell Surface
• Specificity: This monoclonal antibody binds most strongly to the IIa isoforms of CD32 (FcRII), a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FCGR2B
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Fc gamma RII/CD32 Antibody (FCGR2A/479) [Allophycocyanin]

• CD32 antigen
• CD32B
• CD32Fc fragment of IgG, low affinity II, receptor for (CD32)
• CDw32
• Fc fragment of IgG, low affinity IIb, receptor (CD32)
• Fc fragment of IgG, low affinity IIb, receptor for (CD32)
• Fc gamma RII
• Fc gamma RIIb
• FCG2
• Fc-gamma RII-b
• FcgammaRIIb
• fc-gamma-RIIb
• FCGR2
• FcgRII
• FcgRIIB
• fcRII-b
• FcRII-c
• IGFR2
• IgG Fc receptor II-b
• low affinity immunoglobulin gamma Fc region receptor II-b

Background

CD32 is a receptor for the Fc region of complexed or aggregated immunoglobulins gamma. This is a low affinity receptor. It is Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in downmodulation of previous state of cell activation triggered via antigen receptors on B cells (BCR), T cells (TCR) or via another Fc receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FCGR2B