Fc epsilon RI Antibody (AER-37) - BSA Free

Immunohistochemistry-Paraffin: Fc epsilon RI Antibody (AER-37) [NBP1-43278] - Staining of human mast cell in human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.

Flow Cytometry: Fc epsilon RI Antibody (AER-37) [NBP1-43278] - Analysis using the FITC conjugate of NBP1-43278. Staining of normal human peripheral blood cells with Anti-Human IgE PE and Mouse IgG2b K Isotype Control FITC (left) or Anti-Human Fc epsilon Receptor I alpha (FceR1) FITC (right).

Flow Cytometry: Fc epsilon RI Antibody (AER-37) [NBP1-43278] - Staining of normal human peripheral blood cells with Mouse IgG2b kappa Isotype Control Purified (green histogram) or Anti-Human Fc epsilon Receptor I a (FcepsilonR1) Purified (blue histogram) followed by Anti-Mouse IgG Biotin and Streptavidin PE . Cells in the lymphocyte gate were used for analysis.

• Reactivity: Hu
• Applications: Flow, IHC
• Clone: AER-37
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

Fc epsilon RI Antibody (AER-37) - BSA Free Summary

• Description: Novus Biologicals Mouse Fc epsilon RI Antibody (AER-37) - BSA Free (NBP1-43278) is a monoclonal antibody validated for use in IHC and Flow. Anti-Fc epsilon RI Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this antibody was FceRI alpha.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FCER1A
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: The AER-37 antibody has been tested by flow cytometric analysis of peripheral blood leukocytes. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Reactivity Notes

Use in Human reported in scientific literature (PMID:33741501).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for Fc epsilon RI Antibody (AER-37) - BSA Free

• alpha polypeptide
• Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide
• Fc-epsilon RI-alpha
• FcERI
• high affinity immunoglobulin epsilon receptor alpha-subunit
• high affinity immunoglobulin epsilon receptor subunit alpha
• high-affinity, of mast cells, alpha polypeptide

Background

The AER-37 monoclonal antibody reacts with the Fc epsilon RI alpha subunit, an IgE-binding subunit lacking signal-transducing ability. Fc epsilon RI alpha is expressed on mast and basophil cells and is upregulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The Fc epsilon RI complex plays an important role in triggering IgE-mediated allergic reactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fc epsilon RI Antibody (NBP1-43278)(2)

Publications using NBP1-43278

• Wegscheid ML Genetic Risk Factors for Neurodevelopmental Disorders: Insights from hiPSC-Cerebral Organoids Thesis 2022-01-01 (IF/IHC)

Bioinformatics

• Gene Symbol: FCER1A