Fc epsilon RI Antibody (43160) [FITC]

Flow Cytometry: Fc epsilon RI Antibody (MAR-1) [FITC] [NBP1-43941] - Staining of the MC/9 cell line with 0.06 ug of Armenian Hamster IgG Isotype Control FITC (open histogram) or 0.06 ug of Anti-Mouse Fc epsilon Receptor I alpha (Fc epsilon R1) FITC (filled histogram). Total viable cells were used for analysis.

• Reactivity: Mu
• Applications: Flow, ICC/IF
• Clone: 43160
• Clonality: Monoclonal
• Host: Armenian Hamster
• Conjugate: FITC
• Concentration: 0.5 mg/ml

Fc epsilon RI Antibody (43160) [FITC] Summary

• Immunogen: The immunogen for this antibody was FceRI alpha.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Armenian Hamster
• Gene: FCER1A
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry < = 0.125 ug/10^6 cells in 100 ul
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
• Application Notes: The MAR-1 antibody has been tested by flow cytometric analysis of the MC/9 cell line (a mouse mast cell line). This can be used at less than or equal to 0.25 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS (pH 7.2) with 0.1% gelatin
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for Fc epsilon RI Antibody (43160) [FITC]

• alpha polypeptide
• Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide
• Fc-epsilon RI-alpha
• FcERI
• high affinity immunoglobulin epsilon receptor alpha-subunit
• high affinity immunoglobulin epsilon receptor subunit alpha
• high-affinity, of mast cells, alpha polypeptide

Background

The MAR-1 monoclonal antibody reacts with the Fc epsilon RI alpha subunit, an IgE-binding subunit lacking signal-transducing ability. Fc epsilon RI alpha is expressed on mast and basophil cells and is up-regulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The Fc epsilon RI complex plays an important role in triggering IgE-mediated allergic reactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FCER1A