Fc epsilon RI Antibody (2C12-3B6) - Azide and BSA Free

Western Blot: Fc epsilon RI Antibody (2C12-3B6) [H00002205-M01] - Analysis of FCER1A expression in transfected 293T cell line by FCER1A monoclonal antibody (M01), clone 2C12-3B6.Lane 1: FCER1A transfected lysate(29.6 KDa).Lane 2: Non-transfected lysate.

Western Blot: Fc epsilon RI Antibody (2C12-3B6) [H00002205-M01] - Analysis of FCER1A over-expressed 293 cell line, cotransfected with FCER1A Validated Chimera RNAi ( Cat # H00002205-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with FCER1A monoclonal antibody (M01), clone 2C12-3B6 (Cat # H00002205-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: Fc epsilon RI Antibody (2C12-3B6) [H00002205-M01] - FCER1A monoclonal antibody (M01), clone 2C12-3B6 Analysis of FCER1A expression in A-549.

Sandwich ELISA: Fc epsilon RI Antibody (2C12-3B6) [H00002205-M01] - Detection limit for recombinant GST tagged FCER1A is 1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, KD
• Clone: 2C12-3B6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Fc epsilon RI Antibody (2C12-3B6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: FCER1A (AAH05912.1, 1 a.a. ~ 257 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAPAMESPTLLCVALLFFAPDGVLAVPQKPKVSLNPPWNRIFKGENVTLTCNGNNFFEVSSTKWFHNGSLSEETNSSLNIVNAKFEDSGEYKCQHQQVNESEPVYLEVFSDWLLLQASAEVVMEGQPLFLRCHGWRNWDVYKVIYYKDGEALKYWYENHNISITNATVEDSGTYYCTGKVWQLDYESEPLNITVIKAPREKYWLQFFIPLLVVILFAVDTGLFISTQQQVTFLLKIKRTRKGFRLLNPHPKPNPKNN
• Specificity: FCER1A - Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FCER1A
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA and RNAi Validation.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Fc epsilon RI Antibody (2C12-3B6) - Azide and BSA Free

• alpha polypeptide
• Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide
• Fc-epsilon RI-alpha
• FcERI
• high affinity immunoglobulin epsilon receptor alpha-subunit
• high affinity immunoglobulin epsilon receptor subunit alpha
• high-affinity, of mast cells, alpha polypeptide

Background

The IgE receptor plays a central role in allergic disease, coupling allergen and mast cell to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma. The allergic response occurs when 2 or more high-affinity IgE receptors are crosslinked via IgE molecules that in turn are bound to an allergen (antigen) molecule. A perturbation occurs that brings about the release of histamine and proteases from the granules in the cytoplasm of the mast cell and leads to the synthesis of prostaglandins and leukotrienes--potent effectors of the hypersensitivity response. The IgE receptor consists of 3 subunits: alpha, beta (MIM 147138), and gamma (MIM 147139); only the alpha subunit is glycosylated.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FCER1A
• Entrez:
  • 2205(Human)
• OMIM:
  • 147140(Human)
• Uniprot:
  • AAH05912(Human)