FBXW7/Cdc4 Antibody

Western Blot: FBXW7/Cdc4 Antibody [NBP2-89145] - Analysis of FBXW7 on different lysates using anti-FBXW7 antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse spleen tissue Lane 2: Rat lung tissue Lane 3: HepG2

Immunocytochemistry/Immunofluorescence: FBXW7/Cdc4 Antibody [NBP2-89145] - Staining FBXW7 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Flow Cytometry: FBXW7/Cdc4 Antibody [NBP2-89145] - Analysis of SH-SY5Y cells with FBXW7 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: FBXW7/Cdc4 Antibody [NBP2-89145] - Staining FBXW7 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

FBXW7/Cdc4 Antibody Summary

• Description: Novus Biologicals Rabbit FBXW7/Cdc4 Antibody (NBP2-89145) is a polyclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein within human FBXW7/Cdc4 aa 84-327. (SwissProt: Q969H0 Human; SwissProt: Q8VBV4 Mouse; SwissProt: D3Z902 Rat)
• Localization: Cytoplasm. Nucleus.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FBXW7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:500-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FBXW7/Cdc4 Antibody

• AGO
• AGOFBXO30
• Archipelago Homolog
• archipelago, Drosophila, homolog of
• Cdc4
• CDC4FLJ16457
• F-box and WD repeat domain containing 7
• F-box and WD-40 domain protein 7 (archipelago homolog, Drosophila)
• F-box and WD-40 domain-containing protein 7
• F-box protein FBW7
• F-box protein FBX30
• F-box protein SEL-10
• F-box/WD repeat-containing protein 7
• FBW6
• Fbw7
• FBW7hCdc4
• FBX30
• FBX30FLJ11071
• FBXO30
• FBXW6
• FBXW7
• hAgo
• homolog of C elegans sel-10
• SCF(Fbw7)
• SEL10
• SEL-10DKFZp686F23254
• SEL10FBW6

Background

Auxin-inducible degron (AID) technology allows rapid depletion of proteins in animal cells and fungi. Auxin includes chemicals such as IAA (indole-3-aceticacid) and NAA (1-naphtalene acetic acid) and includes inhibitors in plants. For the degradation, and SCF ubiquitin ligase complex containing the TIR1 protein is activated via the association of auxin with TIR1. Because the core SCF components are conserved in all eukaryotic cells, it is possible to transplant the degradation pathway to yeast and mammalian cells by expressing TIR1. In those cells expressing TIR1, a protein fused with degron (AID degron) derived from AUX/IAA can be rapidly degraded in the presence of auxin. Anti-mini-AID tag can detected both mini-AID-tag and full-length AID-tag.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FBXW7